Microcalorimetric Study on the Effect of Sodium Arsenite on Metabolic Activity of Mitochondria Isolated from Carassius auratus Liver Tissue  

作　　者：戴捷 刘义 朱军成 张业中 

机构地区：[1]Department of Environmental Engineering, College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei 434025, China [2]Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei 430072, China

出　　处：《Chinese Journal of Chemistry》2006年第8期997-1000,共4页中国化学（英文版）

基　　金：Project supported by the Research Program of Hubei Provincial Department of Education, China （No. 2004X048）, the National Natural Science Foundation of China （No. 30570015）, and the Teaching and Research Award Program for 0utstanding Young Professors in High Education Institute, the Ministry of Education, China.

摘　　要：Metabolic thermogenic curves of mitochondria isolated from liver tissue of Carassius auratus and the effect of different concentration of NaAsO2 on it were investigated by TAM air isothermal microcalorimeter, ampoule method, at 28.00 ℃. From the thermogenic curves, activity recovery rate constants k, the maximum heat production rate （Pmax） and the total heat produced （Q） were obtained. The values of k and Pmax decline gradually with the increase of the concentration of NaAsO2, and both of the values of Pmax and k are highly correlated to the concentration of NaAsO2. When concentration of NaAsO2 reached 16.0 μg/mL, the maximum heat production rate dropped to 70.8% of the control group, and the corresponding percentage of k was 71.4% of the control group. This experimental result indicates that the addition of NaAsO2 has restrained the metabolic activities of mitochondria in vitro.Metabolic thermogenic curves of mitochondria isolated from liver tissue of Carassius auratus and the effect of different concentration of NaAsO2 on it were investigated by TAM air isothermal microcalorimeter, ampoule method, at 28.00 ℃. From the thermogenic curves, activity recovery rate constants k, the maximum heat production rate （Pmax） and the total heat produced （Q） were obtained. The values of k and Pmax decline gradually with the increase of the concentration of NaAsO2, and both of the values of Pmax and k are highly correlated to the concentration of NaAsO2. When concentration of NaAsO2 reached 16.0 μg/mL, the maximum heat production rate dropped to 70.8% of the control group, and the corresponding percentage of k was 71.4% of the control group. This experimental result indicates that the addition of NaAsO2 has restrained the metabolic activities of mitochondria in vitro.

关 键 词：MICROCALORIMETRY THERMOKINETICS sodium arsenite MITOCHONDRIA 

分 类 号：Q244[生物学—细胞生物学]