DNA-Binding and Cleavage Studies of Zinc（Ⅱ） Mixed-polypyridyl Complex  

作　　者：袁彩霞 魏毅斌 杨频 

机构地区：[1]Institute of Molecular Science. The Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Shanxi University, Taiyuan Shanxi 030006, China

出　　处：《Chinese Journal of Chemistry》2006年第8期1006-1012,共7页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （No. 30470408） and Shanxi Provincial Natural Science Foundation.

摘　　要：The complex of Zn[（phen）（dione）Cl]ClO4·H2O （where phen is 1,10-phenanthroline and dione is 1,10-phenanthroline-5,6-dione） has been synthesized and characterized. The interaction of the complex with DNA was investigated using UV absorption, fluorescence spectroscopy, and electrophoresis measurements. The results show that the complex mainly binds to the double helix of DNA with intercalation mode and the binding constant K is 2.4× 10^4 mol^-1·L. Moreover, the complex can efficiently cleave plasmid DNA at physiological pH and temperature. The cleavage occurs via a hydrolysis mechanism, which is showed by adding radical scavengers, rigorously anaerobic experiments, analysis for malondialdehyde-like products, and the hydrolysis experiment of BDNPP with a rate constant kobs of 5.3 × 10^- 6 s^- 1.The complex of Zn[（phen）（dione）Cl]ClO4·H2O （where phen is 1,10-phenanthroline and dione is 1,10-phenanthroline-5,6-dione） has been synthesized and characterized. The interaction of the complex with DNA was investigated using UV absorption, fluorescence spectroscopy, and electrophoresis measurements. The results show that the complex mainly binds to the double helix of DNA with intercalation mode and the binding constant K is 2.4× 10^4 mol^-1·L. Moreover, the complex can efficiently cleave plasmid DNA at physiological pH and temperature. The cleavage occurs via a hydrolysis mechanism, which is showed by adding radical scavengers, rigorously anaerobic experiments, analysis for malondialdehyde-like products, and the hydrolysis experiment of BDNPP with a rate constant kobs of 5.3 × 10^- 6 s^- 1.

关 键 词：zinc（Ⅱ） complex DNA BINDING hydrolytic cleavage 

分 类 号：O614.241[理学—无机化学]