DNA修复基因XRCC1单核苷酸多态性与肺癌易感性  

POLYMORPHISMS IN THE DNA REPAIR GENE XRCC1 AND SUSCEPTIBILITY TO HUMAN LUNG CANCER

作　　者：余红平[1] 曾小云[1] 仇小强[1] 徐顺清[2] 施侣元[3] 张晓蓉[4] 李媛媛[2] 

机构地区：[1]广西医科大学公共卫生学院,南宁530021 [2]华中科技大学同济医学院环境医学研究所 [3]华中科技大学同济医学院公共卫生学院流行病与卫生统计学系 [4]湖北省肿瘤医院放疗科

出　　处：《广西医科大学学报》2006年第3期355-358,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金重大项目子课题(No.39990570);国家卫生部基金资助项目(3-1)

摘　　要：目的：探讨XRCC1基因单核苷酸多态性与肺癌易感性的关系。方法：以聚合酶链反应-限制性酶切片断多态性方法分析了肺癌病例（n=104）和按性别、年龄频数配比的健康对照者（n=121）XRCC1基因Arg194Trp和Arg399Gln位点的多态性，并比较不同基因型与肺癌风险的关系。结果：XRCC1 Arg399 Arg和Arg399Gln基因型频率在对照组和病例组中的分布差异无统计学意义（P〉0．05）；病例组XRCC1 Gln399Gln突变纯合子基因型频率为13．46％，高于对照组3．31％（P〈0．01）。与携带Arg 399 Arg野生纯合子基因型者比较，携带Gln399Gln突变纯合子基因型个体发生肺癌的风险增加6．11倍（校正OR=6．11，95％CI=1．70-22．0）；携带Arg399Gln突变杂合子基因型者患肺癌风险无显著增加（校正OR=1．03，95％CI=0．60～1.78）。XRCC1 Arg194Arg、Arg194Trp和Trp194Trp基因型频率在对照组和病例组中的分布差异均无统计学意义（P〉0．05）；与携带Arg194Arg野生纯合子基因型者比较，携带至少1个194Trp等位基因者（即Arg194Trp和Trp194Trp基因型）患肺癌风险无显著增加（校正OR：0．998，95％CI=0．97～1.03；校正OR=1.81，95％CI=0．30～11.08）。结论：DNA碱基切除修复基因XRCC1 Gln399Gln突变纯合子基因型者患肺痛风险增加．Objective：To investigate the correlation between XRCC1 genetic polymorphisms and susceptibility to human lung cancer. Methods：A hospital-based case-control study of lung cancer was conducted. The cases （ n = 104） were all newly diagnosed lung cancer in Wuhan, and healthy controls were matched to the cases in frequency on age（±5years）and sex （ n = 121）. XRCC1 genotype was determined from blood-derived DNA using a polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Logistic regression analysis was performed to calculate the adjusted odds ratios （ORs） and 95% confidence intervals （CIs）. Result：For XRCC1 codon 399 genotypes, there is no significant difference between frequencies of the Arg/Arg and Arg/Gln among patients and controls （ P〉0.05）, and Arg/Gln genotypes were not associated with risk （adjusted OR=1.03,95% CI =0.60-1.78） for lung cancer compared with Arg/ Arg genotype. Only the frequency of XRCC1 399 Gln/Gln genotype was higher in the cases （13.46%） than that in controls （3.31%） and associated with an increased risk （adjusted OR= 6.11, 95% CI= 1.70-22.0） for lung cancer compared with Arg/Arg genotype. Conclusion：DNA repair XRCC1 399 Gln/Gln genotype may increase risk of lung cancer.

关 键 词：肺癌 基因多态性 易感性 XRCC1基因 分子流行病学 

分 类 号：R734.2[医药卫生—肿瘤]