高效液相色谱非多孔型阴离子交换柱分析DNA片段  

Analysis of DNA Restriction Fragments by High Performance Liquid Chromatography(HPLC) on a Nonporous Ion Exchange Column

作　　者：廖杰[1] 周建忠[1] 钱小红[1] 董芳霆[1] 刘志红 

机构地区：[1]军事医学科学院国家生物医学分析中心

出　　处：《色谱》1996年第6期462-463,共2页Chinese Journal of Chromatography

摘　　要：建立了高效液相色谱非多孔型阴离子交换柱分离ＤＮＡ片段的方法，用ＴＳＫｇｅｌＤＥＡＥ－ＮＰＲ柱、Ｔｒｉｓ－ＨＣｌ缓冲液（ｐＨ９．０）、１．０ｍｏｌ／ＬＮａＣｌ多阶式线性梯度洗脱，分析了核酸分子量参照物ｐＢＲ３２２ＤＮＡ－ＨａｅⅢ，ＬａｍｂｄａＤＮＡ－ＨｉｎｄⅢ及乙肝病毒基因ＰＣＲ产物，探讨了梯度、流速对分离的影响，方法简便、快速。A rapid and high resolution method for the analysis of DNA restriction fragments by HPLC on a non porous ion exchange column was developed. The analytical column was 35mm×4.6mm i.d. TSK del DEAE NPR, 2 5μm of which the surface was chemically bonded with diethylaminoethyl groups. pBR322 DNA Hae Ⅲ digest, Lambda DNA Hind Ⅲ digest and a kind of PCR product have been separated by gradient elution of sodium chloride in 20mmol/L Tris HCl buffer(pH 9.0). The sma

关 键 词：高效液相色谱法 离子交换柱 DNA片段 

分 类 号：Q523.03[生物学—生物化学]