茶叶中维生素C和嘌呤碱的高效液相色谱测定法的研究  

Determination of Vitamin C and Purine Bases in Tea by High Performance Liquid Chromatography

作　　者：杨志洁[1,2] 叶建农[1,2] 刘彤[1,2] 方禹之[1,2] 

机构地区：[1]云南大理师范高等专科学校化学系 [2]华东师范大学化学系

出　　处：《色谱》1996年第6期492-493,共2页Chinese Journal of Chromatography

摘　　要：报道了采用高效液相色谱法（ＨＰＬＣ）对茶叶中维生素Ｃ、可可碱、茶碱和咖啡碱同时分离检测的方法。碳十八柱为分离柱，柱温４０℃，检测波长２３０ｎｍ，甲醇－水（１０∶９０）为流动相，在１２ｍｉｎ内可全部出峰。方法操作简便，分离效果、重现性和线性范围均令人满意。A method for simultaneous separation and detection of vitamin C as well as several purine bases is described. Determination of these analytes can be accomplished within 12min by using 5μm Hypersil ODS column (200mm×2.1mm i.d.) at 40℃, methanol/H 2O(10∶90) as the mobile phase, followed with DAD detection at 230nm. This method possesses the advantages of easy operation, effective separation, good reproducibility and satisfactory linear range.

关 键 词：高效液相色谱法 维生素C 嘌呤碱 茶叶 

分 类 号：TS272.7[农业科学—茶叶生产加工]