在肽库中进行小肽筛选的初步研究  

A Preliminary Study on the Possibility of Screening Small Peptide in Peptide Library

作　　者：齐杰[1] 方锐[1] 周慧[1] 李惟[1] 沈家骢[1] 

机构地区：[1]吉林大学分子生物系

出　　处：《高等学校化学学报》1996年第11期1738-1741,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：以合成六肽ＤＧＧＳＡＡ为模型对在噬菌体肽库中进行小肽筛选做了初步研究。结果表明，含有可形成氢键和离子键残基的小肽能够在噬菌体肽库中进行筛选，并且明显提高了筛选的专一性。筛选收率由０．００１％降低到０．０００２２％。Peptide libraries have been successfully used in the field concerning molecular interactions,such as antigen-antibody,receptor-ligand,enzyme-inhibitor and so on.These research results show that a few critical residues which can form hydrogen bond or ionic bond in proteins make a major contribution to the interactions of proteins.This paper reports the possibility of screening short peptide in phage hexa-peptide library using DGGSAA as a model.The results show that it is possible to screen the short peptide containing the residues forming hydrogen bond and ionic bond in peptide library,which improved the specificity of screening greatly and the recovery of phage reduced from 0. 001%t0 0.000 22%.That indicates that the interactions between short peptides is strong enough to survive the screening and it supposes a simple model to research molecular recognition with peptide libraries.

关 键 词：肽库 筛选 噬菌体 肽 

分 类 号：Q514.3[生物学—生物化学] Q939.48