正交晶型江浙蝮蛇毒碱性磷脂酶A2初步结晶学研究  

Cystallization and Preliminary Diffraction Data of Orthorhombic Crystal Form of Basic Phospholipase A2 from the Venom of Agkistrodon Halys Pallas

作　　者：孟五一[1] 牛秀田[1] 桂璐璐[1] 林政炯[1] 朱洪[2] 周元聪[2] 李根培[3] 

机构地区：[1]中国科学院生物物理研究所生物大分子国家实验室,北京100101 [2]中国科学院上海生物化学研究所,上海200032 [3]北京大学化学系,北京100871

出　　处：《物理化学学报》1996年第10期946-949,共4页Acta Physico-Chimica Sinica

基　　金：中国科学院资助

摘　　要：The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9. 175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolutiorl using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewermolecules in the asymmetric unit.The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9. 175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolutiorl using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewermolecules in the asymmetric unit.

关 键 词：碱性磷脂酶A2 江浙蝮蛇毒 溶血作用 结晶学 

分 类 号：R996.3[医药卫生—毒理学]