Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction  

作　　者：WANG Jian-an LUO Rong-hua ZHANG Xing XIE Xiao-jie HU Xin-yang HE Ai-na CHEN Jie LI Jia-hui 

机构地区：[1]Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China [2]Center of Lab, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2006年第8期641-647,共7页浙江大学学报（英文版）B辑（生物医学与生物技术）

摘　　要：Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors (ACEIs) perindopril into ischemic myocardium after acute myocardial infarction. Methods: Forty rats were divided into four groups: control, MSC, ACEI, MSC+ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC+ACEI group. Phosphate-buffered saline (PBS) was injected into control group. Perindopril was administered p.o. to ACEI group and MSC+ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson’s trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase (MMP)-2, matrix metalloproteinase (MMP)-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 in infarction area was detected by reverse transcriptase PCR (RT-PCR) analysis. Results: The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group, ACEI group, and MSC+ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4 groups. Conclusion: Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism.Objective： This study was performed to evaluate whether implantation of mesenchymal stem cell （MSC） would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors （ACEIs） perindopril into ischemic myocardium after acute myocardial infarction. Methods： Forty rats were divided into four groups： control, MSC, ACEI, MSC＋ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC＋ACEI group. Phosphate-buffered saline （PBS） was injected into control group. Perindopril was administered p.o. to ACEI group and MSC＋ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson＇s trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase （MMP）-2, matrix metalloproteinase （MMP）-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase （TIMP）- 1 in infarction area was detected by reverse transcriptase PCR （RT-PCR） analysis. Results： The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group, ACEI group, and MSC＋ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4 groups. Conclusion： Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism.

关 键 词：REMODELLING Acute myocardial infarction PERINDOPRIL Bone marrow mesenchymal stem cell 

分 类 号：R54[医药卫生—心血管疾病]