豫医无毛小鼠近交系与HLC小鼠近交系遗传纯度检测  

Detection of genetic homozygosity of YYHL hairless inbred strain and HLC inbred strain

作　　者：薛敬礼[1] 章金涛[1] 杜春燕[1] 邢金山[1] 杜献堂[1] 

机构地区：[1]郑州大学实验动物中心,郑州450052

出　　处：《郑州大学学报（医学版）》2006年第4期665-666,共2页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目39970119;河南省医学创新人才工程基金资助项目20010907

摘　　要：目的:检测豫医无毛小鼠近交系(YYHL)与HLC小鼠近交系的遗传纯合程度。方法:依据GB/T14927.12001检测分布于8条染色体上的13个生化标记基因位点:Car2、Hbb、Es1、Trf、Mod1、Idh1、Es2、Es3、Mup、Pgm1、Gpd1、Ce2、Gpi1;每个品系抽样4只,并用标准近交系C57BL/6、DBA/2、615、BALB/c作对照,对YYHL小鼠近交系和HLC小鼠近交系进行遗传检测。结果:YYHL与HLC小鼠近交系在所检测的13个生化标记基因位点上达到100%纯合。但2个近交系在Hbb、Idh1、Ce2生化标记基因位点上不同。结论:YYHL小鼠近交系与HLC小鼠近交系在遗传纯度方面均已达到近交系要求,可作为新的近交系应用于科学研究。Aim： To detect genetic bomozygosity of YYHL hairless inbred strain. Methods：According to the GB/T 14927.1-2001, thirteen biochemical marker genes （Car-2, Hbb, Es-1, Trf, Mod-1, Idh-1, Es-2, Es-3, Mup, Pgm-1, Gpd- 1 , Ce-2, Gpi-1 ） located on the eight chromosomes were detected in 4 mice from YYHL and HLC inbred strains, respectively. At the same time, the samples from inbred strain C57BL/6, DBA/2, 615, BALB/c mice were detected as control. Results ： Thirteen biochemical marker genes of YYHL and HLC inbred strain were 100% homozygous, but there were 3 different gene loci on biochemical marker Hbb, Idh-1 and Ce-2. Conclusion： YYHL inbred strain and HLC inbred strain are qualified for inbred strain in the heredity purity, and can be applied to scientific research as new inbred strain.

关 键 词：HLC近交系 YYHL近交系 生化标记基因 

分 类 号：R-332[医药卫生]