中国广东人CYP2F1基因遗传多态性研究  

Study on genetic polymorphisms of CYP2F1 gene in Guangdong population of China

作　　者：姜桔红[1] 李智[1] 苏广 贾卫华 张如华 余杏娟 张萌[1] 文剑明[1] 曾益新 

机构地区：[1]中山大学附属第一医院病理科,广州510080 [2]广东省惠东县人民医院 [3]肿瘤防治中心实验研究部

出　　处：《中华医学遗传学杂志》2006年第4期383-387,共5页Chinese Journal of Medical Genetics

基　　金：2005年度国际科技合作重点项目(2004DFA05700)

摘　　要：目的检测广东地区正常人群和鼻咽癌患者中细胞色素P450酶系CYP2F1基因的多态性，并分析该基因遗传多态性与鼻咽癌易感性的关联。方法采用直接测序法检测40例鼻咽癌患者全血标本中CYP2F1基因全部10个外显子的多态性变化。对于等位基因频率较高的多态性位点，进一步采用错配聚合酶链反应．限制性片段长度多态性检测368例鼻咽癌患者和344名正常对照人群中该位点的等位基因频率。结果在40例鼻咽癌样本中，共检测到CYP2F1基因的35个单核苷酸多态性。其中，10个单核甘酸引起编码的氨基酸改变，1个移码突变，15～16bp之间插入C引起移码突变（15-16ins C），该等位基因频率为25％。但病例-对照分析却未能显示该位点突变与鼻咽癌易感的相关性（P〉0．05）。结论中国广东人的CYP2F1基因遗传多态性位点较多，但暂未发现与鼻咽癌的易感性关联的单一多态位点，多个多态性位点或不同基因多态性位点的协同互补作用可能才是鼻咽癌发生发展的关键影响因素。Objective To investigate the genetic polymorphism of CYP2F1 gene, a member of CYP450 gene family in the healthy population and the patients with nasopharyngeal carcinoma （NPC） of Guangdong province, and furthermore analyze the relationship between CYP2F1 genetic polymorphism and the risk of developing NPC. Methods By direct gene sequencing, all of 10 exons of CYP2F1 gene were detected in 40 peripheral blood specimens of patients with primary NPC. For the genetic polymorphism with high allelic frequency, mismatch PCR-RFLP technique was developed to identify the different frequency between 368 NPC cases and 344 cancer-free controls. Results There were totally 35 SNPs identified in all of 10 exosn and exon-intron junctions of CYP2F1 gene from 40 NPC patients, which included 10 missense mutations and 1 frame shift mutation. The most important mutation was C insertion located in 15-16 bp, which caused the flame shift. The allelic frequency of C insertion was 25 %. However, there was no significant difference found between 368 NPC cases and 344 controls in allehc frequency of 15-16 bp C insertion mutation （ P 〉 0.05）. Conclusion A lot of genetic polymorphism of CYP2F1 gene is found in Guangdong population of China. However, no single genetic polymorphism associated with the individual susceptibility to NPC can be identified. The cooperated operations with multiple genetic polymorphisms of one or more genes may be critical factors contributing to the development and progression of NPC.

关 键 词：鼻咽肿瘤 细胞色素P450 CYP2F1基因 遗传多态性 

分 类 号：R394[医药卫生—医学遗传学]