慢性经耳电点燃癫痫对大鼠穿梭箱被动回避反应的记忆保持能力的作用  

Effect of chronic transauricular kindled seizures on passive-avoidance test memory retention in rats

作　　者：李青[1] 刘路英[1] 朱媛媛[1] 周文霞[2] 陈忠[1] 

机构地区：[1]浙江大学医学院药理学教研室,浙江杭州310031 [2]浙江医学高等专科学校生理学教研室,浙江杭州310053

出　　处：《浙江大学学报（医学版）》2006年第4期419-423,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金资助项目(30371638);浙江省青年人才基金项目(R303779);浙江医学高等专科学校自然科学基金项目(2005XZ03)

摘　　要：目的：探讨慢性经耳电点燃癫痫对大鼠穿梭箱被动回避反应的记忆保持能力的影响。方法：大鼠每24h一次经双耳夹给予亚惊厥剂量电刺激（40mA，0．2S）诱发电点燃大发作癫痫，然后利用穿梭箱系统检测大鼠的被动回避反应的记忆保持能力，HE染色法观察海马神经元的病理变化，高效液相色谱分析法检测海马中组胺、γ-氨基丁酸、谷氨酸的含量变化。结果：所有大鼠在连续刺激7～10d后均完全点燃。慢性经耳电点燃癫痫的穿梭箱被动回避反应的记忆保持能力明显下降，海马CA1神经元发生退行性变化，海马内组胺含量下降。结论：慢性经耳电点燃癫痫可导致大鼠被动回避反应记忆保持能力下降，可能与癫痫造成的海马CA1神经元变性及海马内组胺能神经活性降低有关。Objective： To investigate effect of chronic transaurlcu/ar Kindled seizures on passive-avoidance test memory retention in rats. Methods： Chronic transauricular kindled seizures was induced by repeated application of initially subconvulsive electrical stimulation through ear -clip electrodes once every 24 h until the occurrence of 3 consecutive clonic-tonic seizures. A passive avoidance test was used to measure memory retention ability. Morphological changes in neurons of hippocampal CA1 region was examined after HE staining. Histamine,γ-aminobutyric acid （GABA） and glutamate levels in the hippocampus were measured by high performance liquid chromatography （HPLC）. Results： Chronic transauricular kindled seizures impaired passive -avoidance test memory retention in rats. The damaged CA1 neurons were observed and histamine content in the hippocampus markedly decreased 24 h after the end of kindling in the chronic transauricular kindled rats. Conclusion： Chronic transauricular kindled seizure impaired passive -avoidance test memory retention,and it might be due to the damaged CA1 neurons and a decrease of histamine in the hippocampus induced by epilepsy.

关 键 词：癫痫/并发症 记忆障碍/病因学 穿梭箱 疾病模型 动物 

分 类 号：R742.1[医药卫生—神经病学与精神病学]