ALL患儿肿瘤细胞的门冬酰胺合成酶活性研究  

Asparagine synthetase activity in pediatric acute lymphoblastic leukemia

作　　者：江华[1] 顾龙君[1] 薛惠良[1] 汤静燕[1] 陈静[1,1] 潘慈[1] 

机构地区：[1]上海交通大学医学院附属新华医院上海儿童医学中心血液肿瘤科,上海200127

出　　处：《中国当代儿科杂志》2006年第4期272-274,共3页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金资助项目(项目编号:30070795)

摘　　要：目的不同类型急性淋巴细胞性白血病(ALL)对左旋门冬酰胺酶(LAsp)敏感度不完全相同,由于LAsp活性水平与细胞内门冬酰胺合成酶活性负相关,因此研究不同类型ALL患儿白血病细胞内门冬酰胺合成酶活性水平分布情况,有助于临床治疗中合理使用LAsp。方法通过HPLCFLD及蛋白定量等技术,检测28例ALL患儿(免疫酶型TALL7例,B细胞系列ALL21例)白血病细胞内门冬酰胺合成酶活性水平。结果TALL患儿肿瘤细胞内AS活性水平较B细胞系列ALL患儿的显著增高(P<0.05),其中TALL患儿肿瘤细胞内AS活性中位水平为每小时9.3nMAsn/mgprotein,B细胞系列ALL患儿为每小时5.2nMAsn/mgprotein。但无论是在B细胞系列ALL患儿还是在TALL患儿中,肿瘤细胞内AS活性存在多态性分布。结论ALL患儿白血病细胞中门冬酰胺合成酶活性存在多态性分布,总体上TALL比B系ALL白血病细胞中门冬酰胺合成酶活性高。Objective To study the cellular activity of asparagine synthetase in different types of childhood acute lymphoblastic leukemia （ALL）. Methods The cellular activity of asparagine synthetase was detected by HPLC-FLD and Protein measurement in 28 ALL children （7 cases of T-ALL and 21 cases of B-lymphoid lineage ALL ） before chemotherapy. Results The asparagines synthetase activity levels in T-ALL children were significantly higher than those of the B-lymphoid lineage ALL patients, with the median activity level of 9.3 nM Asn/mg protein/hr vs 5.2 nM Asn/mg protein/hr （ P 〈 0.05 ）. The distribution of the asparagine synthetase activity demonstrated a polymorphism in either T-ALL or B-lymphoid lineage ALL patients. Conclusions The cellular activity of asparagines synthetase in ALL patients is presented with a polymorphism distribution. The asparagines synthetase activity levels in T-ALL are significantly higher than in B-lymphoid lineage ALL.

关 键 词：白血病 淋巴细胞性 急性 门冬酰胺合成酶 儿童 

分 类 号：R733.71[医药卫生—肿瘤]