乳腺癌新鲜离体组织的傅立叶变换红外光谱研究  

FTIR spectroscopic characterization of freshly removed breast cancer tissues

作　　者：周苏[1] 徐智[1] 凌晓锋[1] 李庆波[2] 徐怡庄[2] 张莉[2] 赵红梅[1] 王立新[1] 侯宽永[1] 周孝思[1] 吴瑾光[2] 

机构地区：[1]北京大学第三医院普通外科,100083 [2]北京大学化学学院

出　　处：《中华肿瘤杂志》2006年第7期512-514,共3页Chinese Journal of Oncology

基　　金：国家重大基础研究前期研究专项基金资助项目(2002CCA01900)

摘　　要：目的探讨通过测定乳腺新鲜离体组织傅立叶变换红外光谱,区分乳腺癌和良性乳腺组织的可行性及机制。方法联合应用衰减全反射(ATR)探头与中红外光纤、傅立叶变换红外光谱仪,检测乳腺手术中切除的新鲜离体乳腺组织29例。比较良、恶性乳腺组织红外光谱各谱带的峰高和相对峰强。结果共得到红外光谱45张。乳腺癌组织与良性乳腺组织的新鲜离体标本红外光谱有明显差异。代表蛋白质结构的I1640／I1550和I1160／I1120、代表蛋白质与脂类相对含量的I1640／I1460和I1550／I1460、代表脂类结构的I1460／I1400以及代表核酸的I1310／I1240,乳腺癌组织与良性乳腺组织间差异均有统计学意义。结论傅立叶变换红外光谱技术可用于临床判别乳腺癌和良性乳腺组织,且具有快速、方便、无创伤的特点。Objective To identify the FTIR spectroscopic characterization of breast cancer and explore the possibility of application of FTIR in differentiation of malignant and benign breast lesions. Methods FTIR spectra of surgically removed fresh breast tissues were measured by spectrometer equipped with mid-infrared fiber optics and an ATR probe. Peaks in the spectra were measured and relative intensity ratios were calculated and analyzed if there are significant differences between the spectra of malignant and benign breast lesions. Results There were significant differences （ P 〈 0. 05 ） between the spectra of malignant breast cancers and benign breast tissues in the relative intensity ratios of different peaks （ I1640/ I1550 and I1160/I1120 for protein structures;I1640/I1460 and I1550/I1460 for relative content of protein and lipid;I1460/I1400 for lipid structures;I1310/I1240 for nucleic acid）. Conclusion FTIR spectroscopy could be a useful tool in clinical diagnosis of breast cancer.

关 键 词：傅立叶变换红外光谱 乳腺肿瘤 新鲜离体组织 

分 类 号：R737.9[医药卫生—肿瘤]