Roseomonas JS018有机磷农药降解酶的纯化  

Purification of the organophosphorus pesticide degrading enzyme from Roseomonas JS018

作　　者：江玉姬[1] 邓优锦[2] 刘新锐[2] 胡方平[3] 谢宝贵[2] 刘福阳[2] 黎志银[2] 

机构地区：[1]福建农林大学食品科学学院 [2]福建农林大学生命科学学院,福建福州350002 [3]福建农林大学植物保护学院

出　　处：《福建农林大学学报（自然科学版）》2006年第4期421-425,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：农业部948资助项目(2003-T17)

摘　　要：本试验对能高效降解几种有机磷农药菌株JS018的降解酶进行分离和纯化.经分析该降解酶主要位于细胞间质,最适盐析硫酸铵饱和度为60%-70%,粗酶液经硫酸铵沉淀、Sephadex G-100凝胶过滤和DEAE-52柱层析得到较高纯度的酶液.通过SDS-PAGE电泳测定该酶相对分子质量约为37 ku,N末端16个氨基酸残基测序结果为:GAPFQNDVPPACYRFR.The organophosphorus pesticide degrading enzyme produced from strain JS018 was purified by ammonium sulfate precipitation, Sephadex G-100 gel filtration and chromatography on DEAE-52. It mainly located in periplasmic space. The test results showed that the optimal concentration of ammonium sulfate was 60% -70%. The molecular weight of the enzyme was about 37 ku by SDS-PAGE. The N-terminal amino acid sequence was determined as fonows： GAPFQNDVPPACYRFR.

关 键 词：有机磷农药降解酶 电泳 层析 纯化 测序 

分 类 号：Q939.9[生物学—微生物学]