慢性丙型肝炎患者瘦素受体Gln223Arg基因多态性及其与瘦素水平和肝病关系的研究  

Correlations between Gln223Arg polymorphisms in leptin receptor gene and serum leptin levels and development of liver diseases in patients with chronic hepatitis C virus infection

作　　者：张妮[1] 刘正稳[1] 韩群英[1] 曾俊涛[1] 

机构地区：[1]西安交通大学医学院第一附属医院感染科,陕西西安710061

出　　处：《中国现代医学杂志》2006年第14期2132-2136,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金;No.30170842

摘　　要：目的探讨HCV相关慢性肝病患者瘦素受体第223位密码子的基因多态性,分析其与瘦素水平及HCV感染的关系。方法慢性HCV感染者66例,其中慢性丙型肝炎患者54例,HCV相关肝硬化10例,HCV相关肝癌2例;正常对照85例。用聚合酶链反应-限制性片断长度多态性分析(PCR-RFLP)法确定瘦素受体基因多态性,酶联免疫吸附试验法测定血清瘦素水平;分析HCV相关慢性肝病患者瘦素受体基因多态性与血清瘦素水平及临床资料的关联性。结果慢性HCV感染者与正常对照组的瘦素受体Gln223Arg基因多态性发生频率差异未见显著性(χ2检验,χ2=0.451,P=0.796)。慢性丙型肝炎组、HCV相关肝硬化组、HCV相关肝癌与正常对照组的瘦素受体Gln223Arg基因多态性发生频率差异未见显著性(χ2检验,χ2=1.881,P=0.930)。慢性HCV感染组(χ2检验,χ2=0.846,P=0.655)和对照组(χ2检验,χ2=1.595,P=0.451)不同性别间瘦素受体Gln223Arg基因多态性发生频率差异未见显著性。病例组瘦素受体基因的第223位密码子的AG基因型(4.067±3.687)ng/mL和GG基因型(5.667±3.641)ng/mL等位基因的血清瘦素水平差异未见显著性。结论瘦素受体Gln223Arg基因多态性与慢性HCV感染和感染后的结局无相关性。HCV相关慢性肝病患者的瘦素受体Gln223Arg基因多态性可能不影响血清瘦素水平。[Objective] To investigate leptin receptor gene polymorphisms and their correlations with leptin levels and chronic HCV infection. [Methods] Sixty-six patients with chronic HCV infection and eighty-five healthy individuals were studied. Serum levels of leptin were determined by enzyme-linked immunoabsorbent assay （ELISA）. The leptin receptor gene polymorphisms were determined by polymerase chain reaction fragment length polymorphisms assay （PCR-RFLP）. Allele frequencies in patients with HCV infection and controls were assessed and their correlations with serum leptin levels were analyzed. [Results] The genotype frequency of Gln223Arg polymorphism in leptin receptor gene between patients with chronic HCV infection and controls had no significant difference. The genotype frequency of Gln223Arg polymorphism in leptin receptor gene between patients with chronic hepatitis C, HCV associated cirrhosis, HCV associated HCC and controls had no significant difference. The mean serum leptin levels （x±s） in patients with AG genotype and GG genotype were （4.067±3.687） ng/mL and （5.667±3.641） ng/mL, respectively, not significantly different. [Concluions] Gln223Arg polymorphism in leptin receptor gene is not correlated with chronic HCV infection, the outcome of chronic HCV infection.

关 键 词：瘦素受体基因 基因多态性 瘦素 慢性丙型肝炎 

分 类 号：R781.59[医药卫生—口腔医学]