足叶乙甙诱导HL－60急性早幼粒白血病细胞程序化死亡的实验研究  

EtoposideInduced Programmed Cell Death of the Promyelocytic Leukemic HL60 Cells in Vitro

作　　者：吴学宾[1] 陈宝安[1] 黄晓明[1] 高雪芝[1] 金宝翠[1] 高翼之[1] 高峰[1] 胡向阳 

机构地区：[1]南京铁道医学院血液病研究室医学分子实验室

出　　处：《南京铁道医学院学报》1996年第3期152-154,共3页Journal of Nanjing Railway Medical College

基　　金：铁道部卫生科研基金

摘　　要：目的：用不同剂量的ＶＰ－１６诱导急性早幼粒白血病细胞程序化死亡（ＰＣＤ）。方法：通过细胞培养技术获取ＰＣＤ细胞，以ＤＮＡ琼脂糖凝胶电泳确定细胞ＰＣＤ的发生，苔盼蓝拒染法检测细胞活力。结果：不同浓度的ＶＰ－１６在试验条件下于４ｈ内均能诱导ＨＬ－６０细胞发生ＰＣＤ。结论：本实验建立的研究细胞ＰＣＤ的方法可靠。OBJECTIVE:This study was aimed to observe the etoposide (VP16)induced programmed cell death (PCD) of the promyelocytic leukemic HL60 cell.METHODS:HL60 cells were cultured in RPMI 1640 medium containing 20% calf serum.For experiments,the medium was supplemented with VP16.The PCD cells obtained from the culture cells were conformed by DNA electrophoresis and viability of cells with trypan blue test.RESULT:PCD of HL60 cells occurs in different concentration of VP16 within 4 hours.CONCLUSION:Our method is suitable for studying PCD and VP16 has a function to induce PCD.

关 键 词：足叶乙甙 白血病 HL-60 程序化细胞死亡 

分 类 号：R733.71[医药卫生—肿瘤]