硒对人肺癌细胞增殖和生物大分子合成的影响  

Influence of Selenium on the Growth and the Synthesis of DNA,RNA and Protein of Human Lung Cancer Cell Line

作　　者：邵泽叶[1] 杨山钟[2] 李基业[2] 张祖贻[2] 陈真如[2] 

机构地区：[1]南京铁道医学院医学检验系 [2]南京铁道医学院附属医院呼吸科

出　　处：《南京铁道医学院学报》1996年第3期163-165,共3页Journal of Nanjing Railway Medical College

摘　　要：目的：观察硒对人肺腺癌细胞系ＳＰＣ－ＡⅠ生长和ＤＮＡ、ＲＮＡ以及蛋白质合成的影响。方法：在一定浓度的人肺腺癌细胞系ＳＰＣ－ＡⅠ中加入不同浓度的亚硒酸钠，用苔盼蓝染色及同位素掺入法观察和研究其变化，对实验结果进行统计处理。结果：当亚硒酸钠浓度在０．５μｇ／ｍｌ时，能明显刺激肿瘤细胞增殖和ＤＮＡ、ＲＮＡ以及蛋白质合成；但当亚硒酸钠浓度增加至１．０μｇ／ｍｌ和２．０μｇ／ｍｌ时，肿瘤细胞明显受到抑制，同时ＤＮＡ、ＲＮＡ和蛋白质合成也明显下降。结论：硒对人肺癌细胞生长和大分子生物合成有双向作用。OBJECTIVE:To study the effects of selenium on the growth and synthesis of DNA,RNA and protein of SPCAΙ human lung cancer cell line.METHOD:Putting Na 2SeO 3 in different concentrations into human lung cancer line SPCAⅠ(in certain concentration),and observing the results of experiments with coloring and mixing with isotope.RESULT:Sodium selenite in low concentration ( 0.5 μg/ml)stimulates cell proliferation and synthesis of DNA,RNA and protein,but tumor cell growth and the synthesis of DNA,RNA and protein are markedly inhibited when cells are cultured in 1.0μg/ml and 2.0μg/ml sadium selenite.CONCLUSION:Selenium has twoway actions on the growth of human lung cancer cells and synthesis of big molecule for living beings.

关 键 词：肺腺癌细胞系 硒 合成 脱氧核糖核酸 核糖核酸 

分 类 号：R734.2[医药卫生—肿瘤]