氨基乙硫醇修饰金电极直接测定芦丁含量的研究  

Direct Determination of Rutin with Au Electrode Modified by 2-Aminoethanethiol Self-assembled Monolayer

作　　者：徐青[1] 王荣[1] 陆秋艳[1] 叶文博[1] 曹晓卫[1] 吴霞琴[1] 

机构地区：[1]上海师范大学生命与环境科学学院化学系,上海200234

出　　处：《分析化学》2006年第7期971-974,共4页Chinese Journal of Analytical Chemistry

基　　金：上海市教委基金(No.03DZ07);上海师范大学校基金(No.SK200406)资助项目

摘　　要：利用分子自组装技术制备氨基乙硫醇修饰金电极,并采用脉冲伏安法直接测定芦丁的含量.以pH 7.0的乙醇和磷酸盐混合缓冲溶液作底液,芦丁在氨基乙硫醇修饰金电极上于0.19 V （vs.SCE）处呈现一灵敏的氧化电流峰,峰电流和芦丁的浓度在8.0～2.5×102 μmol/L范围内呈良好的线性关系.由于抗坏血酸在氨基乙硫醇修饰金电极上的氧化电位出现显著负移,因此,可避免抗坏血酸对芦丁检测的干扰.本方法可以不经预分离直接检测药物中芦丁含量.A gold electrode modified with 2-aminoethanethiol self-assembled monolayer （SAM） have been used for the directly determination of rutin in the presence of Ascorbic acid（AA） by differential pulse voltammerry. The peak currents at 0. 19 V （vs. SCE） are proportional to the concentration of rutin in the range of 8. 0-2.5 × 10^2μmoL/L. Compared with the oxidation of AA at the bare Au electrode, oxidation potential of AA at the modified electrode negatively shifts 300 mV. The interference of AA during the determination of rutin can be neglected with the 2-aminoethanethiol self-assembhd monolayer modified electrode. A novel method has been proposed for the determination of rutin in medicament without separation.

关 键 词：自组装膜 脉冲伏安法 氨基乙硫醇 芦丁 抗坏血酸 

分 类 号：O657.1[理学—分析化学] TQ124.51[理学—化学]