山楂叶中总黄酮含量的测定  

Determination of the Content of Total Flavones in Hawthorn Leaves

作　　者：王孟歌[1] 田宝娟[1] 乔凤霞[1] 闫宏远[1] 康永胜[1] 

机构地区：[1]保定师范专科学校化学系,河北省保定市071000

出　　处：《光谱实验室》2006年第4期755-757,共3页Chinese Journal of Spectroscopy Laboratory

摘　　要：用索氏提取法提取山楂叶中黄酮化合物。以芦丁为标准品,对其进行络合显色,在最大吸收峰500nm处测定提取物中总黄酮的含量。山楂叶中总黄酮的含量以芦丁计算为9.16%,平均加样回收率为98.53%。方法简便、快速、重现性好,可作为检测山楂叶中黄酮含量的一种手段。The flavones compounds were obtained by soxhlet extraction method. Rutin was reference standard and the concentration of flavones was determined by UV spectroscopy at the wavelength of 500nm after the cxtractant reacted with coloring agents. The flavones content in samples was 9. 16% calculated by rutin,and the recovery rate was 98. 53% respectively. The results show that the method is suitable for simple,rapid and reproducible analysis of flavones in hawthorn leaves.

关 键 词：分光光度法 山楂叶 黄酮 

分 类 号：R15[医药卫生—营养与食品卫生学] O657.32[医药卫生—公共卫生与预防医学]