穴位埋药线对癫痫持续状态大鼠海马神经元凋亡的影响  

Effect of acupoint embedding therapy on apoptosis of hippocampal neurons in rats with status epilepsy

作　　者：陈文华[1] 庄礼兴[2] 丁晓虹[3] 

机构地区：[1]广州中医药大学基础医学院解剖教研室,广东省广州市510405 [2]广州中医药大学针灸推拿学院,广东省广州市510405 [3]解放军广州军区广州总医院康复理疗科,广东省广州市510010

出　　处：《中国临床康复》2006年第31期96-98,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东省中医药局资助项目(111342)~~

摘　　要：目的:观察穴位埋药线对癫痫持续状态大鼠海马神经元形态和神经元细胞凋亡的影响,并分析其发生机制。方法:实验于2002-11-18/24在广州中医药大学针灸推拿学院针灸治疗学实验室完成。①选用健康纯系雄性SD大鼠40只,1.5～2月龄。喂养1周后按随机抽签法将大鼠分为5组:空白组、模型组、西药组、常规针刺组、穴位埋药线组,每组8只。模型组、西药组、常规针刺组、穴位埋药线组大鼠均腹腔注射青毒素4×106IU/kg造成癫痫持续状态模型;空白组腹腔注射与造模组同等剂量的温生理盐水。以大鼠出现行为和皮质脑电图改变判断造模是否成功。具体方法如下:穴位埋药线组:在造模前3d埋线,选取大鼠大椎、心俞透膈俞(双)穴。用医用羊肠线浸泡于安定液24h后,将药线埋植在穴位的皮下组织或肌层内。常规针刺组:选大椎、心俞透膈俞(双)穴,用美容针,平补平泻,30min/次,1次/d,在造模前5d开始治疗,致痫当天也予治疗。西药组:致痫后即予腹腔注射0.25mg/kg苯妥英钠。空白组和模型组不予治疗。②所有动物均在致痫24h后,取脑,制成石蜡切片。以苏木精-伊红染色光镜下观察癫痫持续状态后大鼠海马神经元形态学改变;以原位细胞凋亡检测法检测穴位埋药线对癫痫持续状态后大鼠海马神经元DNA片段化的影响,每张切片计数3个不同视野(×400)内的阳性细胞数,取平均数作为细胞凋亡指数。③计量资料差异比较采用t检验。结果:大鼠40只均进入结果分析。①海马神经元细胞形态:空白组大鼠海马神经元细胞形态正常。致痫后24h,模型组、西药组、常规针刺组和穴位埋药线组的大鼠海马神经元细胞均可见不同程度的凋亡表现。②神经元细胞凋亡情况:模型组细胞凋亡指数明显高于空白组[(85.26±22.76),(11.50±4.20)个,P<0.01];穴位埋药线组、西药组与常规针刺组细胞凋亡指数低于模型组[(25AIM： To study the effect of acupoint embedding therapy on shape of hippocampal neurons and apoptosis of neurons in rats under status epilepsy （SE）, METHODS： The experiment was conducted in the Laboratory of Accupuncture, . Acupuncture and Tuina College of Guangzhou Traditional Chinese Medicine from 18^th to 24^th November 2002. ①Forty healthy. SD male rats with the age of 1.5-2 months and body mass of 160-180 g were selected. Rats were randomly divided into 5 groups after one-week feeding： blank group, model group, western medicine group, routine acupuncture group and acupoint embedding therapy group with 8 rats in each group. Rats in the model group, western medicine group, routine acupuncture group and acupoint embedding therapy group were made into SE models by intraperitoneal injection of penicillin according to 4×10^6 IU/kg. Rats in the blank group were intraperitoneally injected with warm normal saline at the same volume as the model group, The modeling was evaluated by behavior and electrocorticogram, The specific methods were as following. Rats in the acupoint embedding group were embedded with strand at 3 days before modeling, and both of Big Vertebra and Heart Shu points were selected. A surgical chorda chirurgicalis was immersed into the apauvin solution for 24 hours, and the strand was then embedded into the subcutaneous tissues and muscular coat of given points, Rats in the routine acupuncture group were acupunctured at Big Vertebra and Heart Shu with surgical needles once a day and 30 minutes each time with mild reinforcing and attenuating method. Treatment was conducted at 5 days before modeling as well as the day of epilepsy-induction. Rats in the western medicine group were intraperitoneally injected with 0,25 mg/kg diphentoin immediately after epilepsy-induction. Rats in the blank group and model group received no treatments.②All rats were executed at 24 hours after epilepsy to obtain the brain, which was made into paraffin sections. The changes of hippocampal neurons in rats wi

关 键 词：埋药 神经元/针灸效应 针刺穴位 癫痫持续状态/穴位疗法 

分 类 号：R749.1[医药卫生—神经病学与精神病学]