黄芩茎叶总黄酮对正常及肾动脉狭窄模型大鼠血压的影响(英文)  

Effect of total flavones from stem and leaf of Scutellaria baicalensis on the blood pressure of normal and renal arterial stenosis rat models

作　　者：益文杰[1] 张丽敏[1] 佟继铭[1] 

机构地区：[1]承德医学院中药研究所,河北省承德市067000

出　　处：《中国临床康复》2006年第31期179-181,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:黄芩茎叶的主要有效部位黄芩茎叶总黄酮具有改善冠脉血流量、脑血流量、延长凝血及凝血酶原时间、降血脂等作用。目的:观察黄芩茎叶总黄酮对正常大鼠和肾动脉狭窄模型大鼠血压的作用。设计:完全随机对照实验。单位:河北省中药新药研究与开发重点实验室。材料:选择鼠龄为2个月的Wistar大鼠70只,清洁级,体质量(250±50)g,雌雄各半,由中国医学科学院实验动物研究所繁育场提供(动物许可证号:01-3003);黄芩茎叶总黄酮由承德医学院中药研究所植化研究室提供;牛黄降压片由哈药集团世一堂制药厂生产,批号:9802304。方法:实验于1999-01/1999-10在承德医学院中药研究所完成。实验室环境:温度(22±1)℃,相对湿度50%～55%,光照时间:照明12h,黑暗12h。①实验1,黄芩茎叶总黄酮对正常大鼠血压的影响:摸球法随机抽取Wistar大鼠30只,用抽签法将大鼠随机分为黄芩茎叶总黄酮100mg/kgriabaicalen-(x±s,n=10)aHg)组、黄芩茎叶总黄酮50mg/kg组和盐水对照组,每组10只。将大鼠腹腔戊巴比妥钠麻醉后,通过MecLab/4e四道生理仪,描记各组大鼠血压后,分别对3组大鼠灌胃给予黄芩茎叶总黄酮100mg/kg,黄芩茎叶总黄酮50mg/kg及生理盐水(0.5mL/100g)。30min后连续观察各组大鼠血压的变化。②实验2,黄芩茎叶总黄酮对肾动脉狭窄高血压模型大鼠血压的影响:采用抽签法将剩余40只Wistar大鼠随机分为黄芩茎叶总黄酮100mg/kg组、黄芩茎叶总黄酮50mg/kg组、盐水对照组、牛黄降压片组,每组10只。按文献方法对各组大鼠制备慢性肾动脉狭窄型高血压模型,术后2周,每天对黄芩茎叶总黄酮50和100mg/kg组大鼠分别给予黄芩茎叶总黄酮100mg/(kg·d),50mg/(kg·d),牛黄降压片组给予牛黄降压片1200mg/(kg·d),盐水对照组给等容积生理盐水。各组均灌胃给药,给药容积0.5mL/100g,连续用药30天后,测量各组大鼠颈动脉血压。主要观察�BACKGROUND： The effective fraction of stem and leaf of Scutellaria baicalensis, total flavones frem stem and leaf of Scutellaria baicalensis （SSTF） can improve blood flow of coronary artery ahd cerebral blood flow, prolong the time of cruor and prothrombin and decrease blood lipid. OBJECTIVE： To observe the effect of SSTF on blood pressure of normal rats and rat models of renal artery stenosis. DESIGN： A completely randomized and controlled experiment. SETTING： Institute of Traditional Chinese Medicine, Chengde Medical College （Key Laboratory of Research and Exploiture of Traditional Chinese Medicine in Hebei Province） MATERIALS： Totally 70 Wistar rats, aged 2 months old , of clean degree, with body mass of （250±50） g, male and female in half, with permission number of 01-3003 , were provided by the Experimental Animal Research Institute, Chinese Academy of Medical Sciences. SSTF was provided by the phytochemical laboratory of Institute of Traditional Chinese Medicine, Chengde Medical College; Niuhuangjiangya plan was produced by Shiyitang Pharmaceutical Factory, Hayao Company （batch number： 9802304）. METHODS： The experiment was conducted in Institute of Traditional Chinese Medicine, Chengde Medical College from January to October 1999. The rats were raised in the environment of （22±1）℃, with relative humidity of 50%-55%, of daylight and darkness for 12 hours for each.① In the experiment 1, we studied the effect of SSTF on the blood pressure of normal rats. Totally 30 Wistar rats were randomly divided into SSTF group of 100 mg/kg （n=10, 100 mg/kg SSTF）, SSTF group of 50 mg/kg （n=10, 50 mg/kg SSTF）, and normal saline control group （n=10, 0.5 mL /100 g normal saline）. Before being administrated, rats were anaesthetized by intraperitoneal injection of pentobarbital sodium. Blood pressure was recorded by MecLab/4e four-channel physiologic instrument. Then intragastric administration of SSTF and normal saline were performed on the rats in SSTF groups and normal salin

关 键 词：黄芩 黄酮类 血压 大鼠 

分 类 号：R749.611[医药卫生—神经病学与精神病学]