电针任脉腧穴对脑缺血大鼠海马星形胶质细胞的调节效应  

Effect of electroacupuncture at Ren Channel and Shu acupoint on astrocytes in hippocampus of rats with cerebral ischemia

作　　者：于海波[1] 杨卓欣[1] 王玲[1] 皮敏[1] 饶晓丹[1] 

机构地区：[1]深圳市中医院针灸科,广东省深圳市518033

出　　处：《中国临床康复》2006年第31期93-95,F0003,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(30371808)~~

摘　　要：目的:观察电针任脉腧穴对于局灶性脑缺血大鼠损伤侧海马星形胶质细胞的转归是否有特异性的正面影响。方法:实验于2004-06/2005-06在中山大学北校区人体解剖教研室完成。Wistar雄性大鼠42只,以随机数字表随机分为假手术组(n=6)、电针组(n=18)和模型组(n=18)。①假手术组:麻醉后仅暴露右侧颈总动脉,不插入尼龙线,手术后不针刺。②电针组:制备大脑中动脉闭塞模型,2h后再灌注。造模成功后第2天开始施加电针任脉腧穴。③模型组:造模方法同电针组,造模成功后并不针刺。各组大鼠相应于造模后7,14,28d麻醉下处死取脑。在激光共聚焦显微镜(OlympusFV500)下观察并计算各组大鼠损伤侧海马胶质纤维酸性蛋白/神经元特异性烯醇化酶免疫荧光双标及胶质纤维酸性蛋白、神经元特异性烯醇化酶阳性细胞。结果:实验动物42只均进入结果分析,中途无脱落。①电针组与模型组造模后7d的胶质纤维酸性蛋白/神经元特异性烯醇化酶荧光双标及胶质纤维酸性蛋白阳性细胞数并无差异(P>0.05),假手术组胶质纤维酸性蛋白阳性细胞数明显较少(P<0.01),3组神经元特异性烯醇化酶双标细胞数两两相比,差异均无统计学意义(P>0.05)。②造模后14d,电针组的胶质纤维酸性蛋白/神经元特异性烯醇化酶荧光双标细胞数明显多于模型组(P<0.01),而胶质纤维酸性蛋白细胞数则明显少于模型组(P<0.01),两组神经元数量差异并无统计学意义(P>0.05)。③造模后28d,电针组神经元特异性烯醇化酶阳性细胞数及胶质纤维酸性蛋白/神经元特异性烯醇化酶荧光双标细胞数均明显较模型组多(P<0.01),而胶质纤维酸性蛋白细胞数则明显较模型组少(P<0.01)。④假手术组可见散在分布的胶质纤维酸性蛋白阳性的星形胶质细胞,胞体较小,突起细长;模型组与电针组缺血侧海马均可见大量胶质纤维酸性蛋白呈强阳性的星形胶质细AIM： To explore if electroacupuncture at Ren Channel and Shu acupoint has special positive effect on astrocytes in the injured hippocampus of rats with cerebral ischemia. METHODS： The experiment was conducted in the Department of Human Anatomy, North Part of Sun Yat-sen University between June 2004 and June 2006. Totally 42 male Wistar rats were selected and randomly divided into sham-operation group （n=6）, electreacupuncturegroup （n=18） and model group （n=18）. ①The sham-operation group： The right common carotid artery was exposed after anesthesia without inserting nylon hne, and the rats were not given acupuncture after operation.②The electreacupuncture group： Cerebral reperfusion ischemic animal models were made by blocking the middle cerebral artery of wistar rats 2 hours before reperfusion. Two days later, the rats were given electreacupuncture at Ren Channel and Shu aeupoint.③The model group： The models were established with the same method of the electreaeupuneture group but not given the acupuncture. The brains of rats were taken under anesthesia on the day 7, 14 and 28, respectively after modeling to observe and calculate the positive ceils of glial fibrillary acidic protein （GFAP）/neurenspecifie enolase （Nse） double-immunofluoreseenee staining, GFAP and Nse in injured hippoeampus of rats in each group by using laser scanning eonfocal microscope （Olympus FV 500）. RESULTS： All the 42 rats were involved in the result analysis without loss in midway. ①After 7 days of modeling, there was no difference in GFAP/Nse double-immunostaining positive cell and GFAP positive cells between the eleetreaeupunture group and model group （P 〉 0.05）, and the number of GFAP positive ceils was less in the sham-operation group （P 〈 0.01）, there was no significant difference in Nse immunofluoreseenee positive ceils among the three groups （P 〉 0.05）. ②After 14 days of modeling, the number of GFAP/Nse double-immunostaining positive cells of the eleetreaeupuneture

关 键 词：穴位 任脉 脑缺血 海马 针刺 电针 

分 类 号：R742[医药卫生—神经病学与精神病学]