解毒通络保肾胶囊保护糖尿病大鼠肾脏及影响结缔组织生长因子mRNA的表达效应(英文)  

Jiedu tongluo baoshen gelatin capsule for protecting the kidney of rats with diabetes mellitus and its effect on the expression of connective tissue growth mRNA

作　　者：赵贤俊[1] 李才[2] 南征[3] 邓悦[4] 

机构地区：[1]延边大学医院中西医结合科,吉林省延吉市133000 [2]吉林大学再生医学科学研究所,吉林省长春市130021 [3]长春中医学院中医研究所,吉林省长春市130021 [4]长春中医学院附属医院内二科,吉林省长春市130021

出　　处：《中国临床康复》2006年第31期174-178,F0003,共6页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:结缔组织生长因子对肾细胞的分化、增生及细胞外基质的合成和降解具有很强的调控作用,其异常表达在各种类型肾脏疾病包括糖尿病肾病纤维化进程中起着至关重要的作用。目的:观察解毒通络保肾胶囊对糖尿病大鼠肾脏病变的保护作用及其对肾脏结缔组织生长因子mRNA表达的影响。设计:以动物为观察对象,随机对照实验。单位:长春中医学院中医研究所,吉林大学再生医学科学研究所病理研究室。材料:选用清洁级Wistar大鼠46只,雌雄各半,体质量150～200g。解毒通络保肾胶囊由吉林省中医院制剂室提供(批号:2002101,由人参、黄芪、生地、枸杞子、知母、地骨皮、黄连、榛花、金银花、大黄、丹参、益母草等药物组成,0.25g/粒);苯那普利(北京诺华制药有限公司提供,批号01046)。方法:实验于2002-05/2003-01在吉林大学再生医学科学研究所病理研究室完成。①选用Wistar大鼠46只,按随机数字表法分为4组:正常对照组(10只),模型组(12只),苯那普利治疗组(12只),中药治疗组(12只)。正常对照组:腹腔内注射等体积枸橼酸钠缓冲液;其余大鼠单次腹腔注射链脲佐菌素50mg/kg,临用前用0.1mol/L枸橼酸缓冲液溶解,pH4.2。造模72h后,尾静脉采血测空腹血糖并同时测尿糖,血糖≥16.7mmol/L,尿糖以上者为糖尿病模型造模成功。苯那普利治疗组:灌胃苯那普利混悬液15mg/(kg·d);中药治疗组:灌胃解毒通络保肾胶囊混悬液5g/(kg·d);正常对照组和模型组每日上午灌胃等体积自来水,1次/d,共12周。②用药12周后检测血糖、尿素氮、血肌酐、尿白蛋白排泄率;用放免法检测肾组织血管紧张素Ⅱ;免疫组化法检测肾脏纤维连接蛋白和Ⅳ型胶原蛋白表达水平;应用反转录聚合酶链反应检测肾皮质结缔组织生长因子基因表达,并进行肾脏组织学观察。③组间比较采用方差分析。主要观察指标:各组大鼠血和尿BACKGROUND： Connective tissue growth factor （CTGF） has very strong regulative effect on the differentiation and proliferation of renal cells as well as the synthesis and degradation of extraeellular matrix. Its abnormal expression plays a key role in the process of fibrous degeneration of various renal diseases including diabetic nephropathy. OBJECTⅣE： To observe the protective effect ofjiedu tongluo baoshen gelatin capsule on renal disease in rats with diabetes mellitus and its effect on the expression of renal CTGF mRNA. DESIGN： A randomized controlled experiment taking animals as subjects. SETTING： Institute of Traditional Chinese Medicine, Changchun College of Traditional Chinese Medicine; Department of Pathology, Institute of Regenerated Medical Sciences, Jilin University MATERIALS： Totally 46 Wistar rats, of clean degree, male and female in half, weighing 150 to 200 g, were used in the experiment. Jiedu tonghro baoshen gelatin capsule was provided by Preparation Room, Traditional Chinese Medicine Hospital of Jilin Province （Batch No. 2002101, composed of Renshen, Huangqi, Shengdi, Gouqizi, Zhimuv Digupiv Huanglian, Zhenhua, Jinyinhua, Dahuang, Danshen, Yimucao and so on, 0.25 g/pill） and benazepril （Provided by Beijing Nuohua Pharmaceutical Company, Batch No. 01046） were used in the experiment. METHODS： This experiment was carried out in the Department of Pathology, Institute of Regenerated Medical Sciences, Jilin University from May 2002 to January 2003. ① Totally 46 Wistar rats used in the experiment were randomly divided into 4 groups： normal control group （n=10）, model group （n=12）, benazepril-treated group （n=12） and traditional Chinese medicine-treated group （n=12）.Rats of normal control group were intraperitoneally injected of natrium citrieum buffer solution of same volume; Rats of other groups were intraperitoneally injected of 50 mg/kg streptozotoein which was dissolved by 0.1 mol/L citric acid buffer solution before use（pH 4.2）. Seventy-two hours

关 键 词：糖尿病 糖尿病肾病/中药疗法 结缔组织 生长物质 解毒剂(中药) 

分 类 号：R781.64[医药卫生—口腔医学]