Toll样受体4(896A/G)单核苷酸多态性与急性胰腺炎相关性的研究  

Relationship between Toll-like receptor 4(896A>G) polymorphisms and acute pancreatitis

作　　者：高宏凯[1] 张新国[1] 周总光[2] 李园[2] 陈友岱[2] 陈友琴[2] 严文伟[2] 

机构地区：[1]武警总医院普外科,北京100039 [2]四川大学华西医院普外科消化外科研究室,成都610041

出　　处：《武警医学》2006年第7期487-490,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家杰出青年科学基金项目(No.39925032)

摘　　要：目的研究Toll样受体(TLR)4(896A>G)在急性胰腺炎(AP)患者和正常人群中的分布及其与AP的相关性。方法采用错配PCR-RFLP分析方法检测AP患者和健康自愿者人群TLR4(896A>G)等位基因频率。结果(1)所有TLR4(896A>G)的基因突变均为杂合子;(2)与健康志愿者组(5.6%)相比,轻型急性胰腺炎(MAP)组TLR4G等位基因频率(9.8%)呈轻度升高(差异无显著性,P=0.182);(3)与健康志愿者组(5.6%)相比,胰腺组织坏死+感染的重型急性胰腺炎(SAP)组TLR4G等位基因频率(20%)明显升高(差异有显著性,P=0.019)。结论TLR4错义突变(896A>G)在AP的发生发展过程中具有双刃性作用:(1)AP早期为一种保护因素;(2)AP后期表现为一种危险因素,TLR4(896A>G)的单核苷酸多态性可能与SAP存在相关性。Objective To study the relationship between Toll- like receptor 4 （ 896A 〉 G ） polymorphisms and acute pancreatitis. Methods TLR4（ 896A 〉 G ）mutation was assayed by mispaifing PCR - RFLP analysis in AP pantents and healthy volunteers. Results （1） All individuals with Toll - like receptor 4 mutations were heterozygous for the TLR4 （ 896A 〉 G ）. （2）Compared with healthy volunteers （5.6%）, the G allele frequency of the TLR4 increased slightly in patients with mild acute pancreatitis （9.8%） without significant difference （ P = 0.182）. （3）Compared with healthy volunteers （5.6%）, significant difference was found in infection of pancreatic necrosis （20%） in severe AP patients （ P =0.019）.Conclusions Human TLR4 （ 896A 〉 G ） mutations have the bidirectional role in the pathogenesis of AP, i. e. protective function in the early stage of AP and risk factor in the later stage of AP.

关 键 词：TOLL样受体4 单核苷酸多态性 急性胰腺炎 

分 类 号：R576[医药卫生—消化系统] R394[医药卫生—内科学]