阿扑吗啡阻抗6-羟基多巴胺毁损纹状体的实验研究  

Apomorphine against 6-OHDA-induced striatal-lesion rat model of Parkinson′s disease

作　　者：袁红[1] 梁立武[2] 张韶峰[3] 许建阳[1] 王梅康[1] 曹力[4] 

机构地区：[1]武警总医院中西医结合康复理疗科,北京100039 [2]武警总医院医务部,北京100039 [3]武警总医院科训科,北京100039 [4]武警总医院感染控制科,北京100039

出　　处：《武警医学》2006年第7期504-506,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨阿扑吗啡是否对6-羟基多巴胺毁损纹状体帕金森病鼠模型有神经保护作用。方法6-羟基多巴胺毁损大鼠左侧纹状体,在毁损前15 min阿扑吗啡(10 mg/kg,皮下注射),连续注射11 d。毁损2周后,分别进行行为学(苯丙胺引起的旋转数目)和神经化学(高压液相测定纹状体多巴胺及代谢物含量)的研究。结果阿扑吗啡能降低苯丙胺引起的向损伤侧旋转的数目。而且,显著减低多巴胺的损耗使其恢复到正常,并使DOPAC/DA比率恢复到正常。结论在6-羟基多巴胺毁损纹状体模型中,阿扑吗啡不仅改善运动功能,而且,恢复纹状体的多巴胺含量。Objective To investigate the possible neuroprotective effect of dopamine （DA） receptor agonist R- apomorphine （R- APO） on the 6 - hydroxydopamine （6 - OHDA） - induced rat model of Parkinson＇ s disease. Methods The rats received an injection of 6 - hydroxydopamine （6 - OHDA） into the left striatum. R - APO administration （ 10 mg/kg, s.c. ） started 15 min before 6 - OHDA injection and was repeated daily for 11 days post- injury to striatum by the injection. Behavioral test was carried out 2 weeks post - injury. Motor impairment was evaluated by amphetamine- induced rotation （5 mg/kg i.p）. The degree of the injury was determined post- mortem by determination of DA and DOPAC contents in the striatum. Results R- APO treatment attenuated the amphetamine- induced ipsiversive rotation and significantly alleviated the 6 - OHDA - induced striatal DA depletion and DOPAC/DA ratios were normalized. Conclusion In the rat model of Parkinson＇s disease, R- APO not only significantly improves motor function but also restores DA content in the striatum.

关 键 词：阿扑吗啡 神经保护6-羟基多巴胺 行为学 多巴胺 帕金森病 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]