干燥性角结膜炎患者局部应用0.1%强的松龙滴眼可减弱神经生长因子表达  

Topical 0.1% prednisolone lowers nerve gr-owth factor expression in keratoconjunctivitis sicca patients

作　　者：Lee H. K. Ryu I. H. Seo K. Y. E.K. Kim 曹绪胜(译) 

机构地区：[1]不详 [2]Department of Ophthalmology, Yonsei University, YongDong Severance Hospital, 146-92 Dokok-dong, Kangnam-Gu, Seoul 135 - 720, SouthKorea

出　　处：《世界核心医学期刊文摘（眼科学分册）》2006年第6期44-45,共2页Digest of the World Core Medical Journals:Ophthalmology

摘　　要：Purpose: To compare nerve growth factor (NGF) levels in tears and on the ocular surfaces of normal control and non-Sj gren’ s type keratoconjunctivitis sicca subjects,and to investigate the effect of 0.1% prednisolone eyedrops on NGF levels in keratoconjunctivitis sicca patients. Design: Prospective,double-masked,randomized,comparative clinical trial. Participants: Forty-one keratoconjunctivitis sicca patients and 23 age-and gender-matched healthy subjects. Methods: Baseline tear NGF levels were measured in keratoconjunctivitis sicca patients and healthy control subjects using enzyme-linked immunosorbent assays. Keratoconjunctivitis sicca patients received 0.1% prednisolone drops in one eye and 0.1% hyaluronic acid drops in the other,3 times a day for 28 days. Also,impression cytology (IC) and immunostaining for NGF on conjunctival epithelium were performed on both groups. Main Outcome Measures: Tear NGF/total tear protein (TP)-concentration ratio,IC and NGF immunocytologic staining,subjective symptom scale,tear breakup time,and Schirmer values. Results: Keratoconjunctivitis sicca patients were found to have baseline tear NGF concentrations higher than those of age-and gender-matched healthy control subjects (65.9± 14.5 vs. 122.1± 45.3 pg/μ g,P<0.0001). In keratoconjunctivitis sicca patients,prednisolone treatment for 28 days resulted in a decrease in tear NGF levels,symptom scores,and IC scores,whereas hyaluronic acid treatment had no such effect (68.2± 25.0 pg/μ g vs. 108.0± 43.4 pg/μ g,P< 0.0001 for tear NGF/TP ratio; 2.16± 1.01 vs. 3.39± 1.50,P=0.0014 for symptom scale; 1.05± 0.67 vs. 1.61± 0.86,P=0.0317 for IC). Measurements taken at both 14 and 28 days indicated that neither prednisolone nor hyaluronic acid treatment affected breakup time or Schirmer values. Conclusion: Keratoconjunctivitis sicca patients showed elevated levels of tear NGF,which were decreased by treatment with 0.1% prednisolone. These data suggest that ocular surface NGF may play an important role in ocular surface inflammaPurpose： To compare nerve growth factor （NGF） levels in tears and on the ocular surfaces of normal control and non-Sjoegren＇s type keratoconjunctivitis sicca subjects, and to investigate the effect of 0. 1% prednisolone eyedrops on NGF levels in keratoconjunctivitis sicca patients. Design： Prospective, double-masked, randomized, comparative clinical trial. Participants： Forty-one keratoconjunctivitis sicca patients and 23 age-and gender-matched healthy subjects. Methods： Baseline tear NGF levels were measured in keratoconjunctivitis sicca patients and healthy control subjects using enzyme-linked immunosorbent assays. Keratoconjunctivitis sicca patients received 0. 1% prednisolone drops in one eye and 0. 1% hyaluronic acid drops in the other, 3 times a day for 28 days. Also, impression cytology （IC） and immunostaining for NGF on conjunctival epithelium were performed on both groups. Main Outcome Measures： Tear NGF/total tear protein （TP） - concentration ratio, IC and NGF immunocytologic staining, subjective symptom scale, tear breakup time, and Schirmer values. Results： Keratoconjunctivitis sicca patients were found to have baseline tear NGF concentrations higher than those of age-and gender-matched healthy control subjects （65.9 ± 14.5 vs. 122. 1 ±45.3 pg/μg, P 〈0. 0001 ）.

关 键 词：神经生长因子(NGF) 干燥性角结膜炎 强的松龙 患者 局部应用 酶联免疫吸附法 SJOEGREN 正常对照组 眼液点眼 减弱 

分 类 号：R777.31[医药卫生—眼科]