中药复方肝癌宁对肝癌细胞生长和AFP分泌的抑制作用  

Inhibitory effect of composite Gan'aining on growth and alpha-fetoprotein secretion of human hepatoma cells

作　　者：袁冬生[1] 王新华[2] 刘东辉[1] 朱彩平[3] 石磊[3] 

机构地区：[1]广州中医药大学职业技术学院,广东省广州市510405 [2]广州中医药大学校长办公室,广东省广州市510405 [3]广州中医药大学05级硕士生,广东省广州市510405

出　　处：《世界华人消化杂志》2006年第19期1912-1915,共4页World Chinese Journal of Digestology

基　　金：广东省科技厅科技计划资助项目;No.2005B33001032

摘　　要：目的：研究中药复方肝癌宁对人肝癌细胞体内、外生长及其对甲胎蛋白(AFP)分泌的影响．方法：以小鼠移植人肝癌模型观察药物(每天10,5,2．5 g/kg)的体内抗肝癌作用．以MTT法测定复方肝癌宁(45 g/L)的体外抗肝癌活性,以ELISA检测AFP分泌量．结果：10 g/kg对小鼠移植人肝癌HepG_2移植瘤的生长有明显抑制作用,抑瘤率为54．5% (P＜0．01),且呈量效关系(r=10,P＜0．01)．与空白对照组相比,复方肝癌宁45 g/L作用4,5,6 d时体外培养的人肝癌HepG_2细胞株的生长明显抑制(7．0±1．3 vs 10．3±0．9,P＜0．05；12．1±0．4 vs 21．3±0．9,P＜0．01；15．2±0．3 vs 45．6±0．6,P＜0．01),AFP分泌量也明显减少(39．6±1 vs 54．5±3；20．8±2 vs 45．6±4；18．5±2 vs 40．2±3：均P＜0．01)．结论：中药复方肝癌宁能明显的抑制肝癌HepG_2细胞生长及AFP分泌水平．AIM： To investigate the effects of composite Gan＇aining （CGN） on the growth and alphafetoprotein （AFP） secretion of human hepatoma cells. METHODS： Mice bearing human hepatoma were treated with CGN （10, 5, and 2,5 g/kg per day）, and the tumor growth was observed, In vitro inhibitory effect of CGN （45 g/L） was detected by MTT assay, and the secretion of AFP was measured by enzyme-linked immunosorbent assay （ELISA）. RESULTS： CGN inhibited the growth of the transplanted hepatoma in a dose-dependent manner （r= 0, P〈0.01）, and the tumor inhibition rate was 54.5% （P〈0.01） when 10g/kg CGN was used. In comparison with that in the controls, the growth of HepG2 cells was significantly inhibited at 4, 5 and 6 d （7.0±1.3 vs 10.3±0.9, P 〈 0.05; 12.1±0.4 vs 21.3±0.9, P〈0.01; 15.2±0.3 vs 45.6±0.6, P 〈 0.01）, and the secretion of AFP was decreased （39.6±1 vs 54.5±3; 20.8±2 vs 45.6±4; 18.5±2 vs 40.2±3; all P〈0.01）. CONCLUSION： CGN can inhibit the growth of hepatoma cells HepG2 in vitro and in vivo, and also decrease AFP secretion of HepG2 cells.

关 键 词：复方肝癌宁 肝肿瘤 甲胎蛋白 

分 类 号：R735.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]