结肠癌组织中p53和凋亡相关基因bcl-2、bax的表达及其临床意义  

Study on Expressions and Clinical Significance of p53 and Apoptosis-associated Genes bcl-2 and bax by Tissue Microarray in Human Colon Cancer

作　　者：陈卫昌[1] 林茂松[1] 张宝峰[2] 方静[2] 周琼[2] 胡莺[2] 郜恒骏[2] 

机构地区：[1]苏州大学附属第一医院消化内科,215006 [2]生物芯片上海国家工程研究中心上海芯超生物科技有限公司

出　　处：《胃肠病学》2006年第7期395-399,共5页Chinese Journal of Gastroenterology

基　　金：国家863计划功能基因组与生物芯片重大专项(No.2002AA2Z2021);江苏省"六大人才高峰"基金(D类)资助

摘　　要：背景:组织芯片已广泛应用于恶性肿瘤的相关研究,但用于研究相关癌基因在结肠癌中表达变化的报道尚较少。目的:研究结肠癌、结肠腺瘤和癌旁结肠黏膜组织中p53和凋亡相关基因bcl-2、bax的表达及其临床意义。方法:取85例结肠癌、18例结肠腺瘤和9例癌旁结肠黏膜组织分别制成72点和104点两块组织芯片,以免疫组化方法检测芯片中p53、bcl-2和bax的表达。结果:p53、bcl-2和bax在结肠癌、结肠腺瘤和癌旁结肠黏膜组织中的表达有显著差异(P<0.01),p53、bcl-2在结肠癌中的表达高于结肠腺瘤和癌旁结肠黏膜组织,bax在结肠癌中的表达低于结肠腺瘤和癌旁结肠黏膜组织。p53和bax在不同组织学分化程度结肠癌中的表达有显著差异(P<0.01,P<0.05),但两者之间无相关性(rs=-0.081,P>0.05),p53与bcl-2的表达呈正相关(rs=0.245,P<0.01)。bcl-2的表达与结肠癌临床病理参数无关。结论:p53异常表达可能是结肠癌发生中的较晚期事件,bcl-2高表达和bax低表达可能参与了结肠癌的形成过程。bax低表达的结肠癌细胞更具有恶性分化潜能。Background：Tissue microarray has been widely used for malignant tumor investigation, but its use in the changes of the related oncogenes in colon cancer is still rare. Aims： To study the expressions and clinical significance of p53 and apoptosis-associated genes bcl-2, bax in colon cancer, colon adenoma and colon para-cancerous mucosa. Methods： Eighty-five cases of colon cancer, 18 cases of colon adenoma and 9 cases of colon para-cancerous mucosa were made into two pieces of tissue microarray containing 72 dots and 104 dots, respectively. Expressions of p53, bcl-2 and bax proteins were detected by immunohistochemical staining in these tissue microarrays. Results： Significant differences were found among colon cancer, colon adenoma and colon para-cancerous mucosa according to the expressions of p53, bcl-2 and bax （P〈0.01）. Expressions of p53 and bcl-2 in colon cancer were higher than those in colon adenoma and colon para-cancerous mucosa while the expression of bax in colon cancer was decreased. A significant difference of expressions of p53 and bax in different histological grades （P〈0.01, P〈0.05） was found in colon cancer. Expression of p53 was positively correlated with bcl-2 （rs=0.245, P〈0.01）, but not with bax （rs=-0.081, P〉0.05） in these cohort tissues. The expression of bcl-2 was not correlated with the clinicopathologieal parameters. Conclusions： Over-expression of p53 may be a late event involved in the development of colon cancer and high expression of bcl-2 and low expression of bax may be correlated with the genesis in colon tumor. Colon cancer with low expression of bax may possess potential malignant differentiation.

关 键 词：结肠肿瘤 基因 p53 细胞凋亡 免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤]