α-^(32)P-dGTP的酶法制备  

THE ENZYMATIC PREPARATION OF α-^(32)P-dGTP

作　　者：吴冯波[1] 王翌善[1] 仪明光 

机构地区：[1]中国原子能科学研究院

出　　处：《同位素》1996年第3期140-144,共5页Journal of Isotopes

摘　　要：以γ－３２Ｐ－ＡＴＰ作磷酸供体，经三步连续的酶反应制备α－３２Ｐ－ｄＧＴｐ。相对于投料的３２Ｐｉ（Ｎａ３３２ＰＯ４）量，产品收率达９３％。分离后的标记物经高效液相色谱（ＨＰＬＣ）测淀，放射化学纯度为９７％。标记物比活度为１．６×１０１７Ｂｑ／ｍｏｌ。A three-step enzyme method is proposed to prepers α-32P-dGTP with γ-32P-ATP as phosphatedonor, involving a series of enzymatic reactions. The yield ofα-32P-dGTP is 93% hased on 32Pi andthe radio chemical purity of the separated α-32P-dGTP is 97% measured by HPLC. The speCific activity of α-32P-dGTP is 1. 6 ×1017 Bq/mol.

关 键 词：α-^(32)P-dGTP 酶法制备 HPLC 

分 类 号：R817[医药卫生—影像医学与核医学]