基因工程法生产降钙素的研究进展  

Advances in production of calcitonin by gene engineering

作　　者：董丹[1] 刘昱辉[2] 吴元华[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110161 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《沈阳药科大学学报》2006年第8期547-552,共6页Journal of Shenyang Pharmaceutical University

基　　金：国家高技术研究与发展计划(863计划)重大专项项目(No.2002AA206511)

摘　　要：目的综述基因工程法生产降钙素的研究进展,为进一步研究利用降钙素提供参考。方法依据国内外刊物公开发表的36篇文献,回顾了降钙素表达系统的研究进展。结果目前基因工程法生产降钙素的表达系统主要有5种:大肠杆菌、乳酸杆菌、酵母、昆虫杆状病毒和植物。结论5个表达系统各有优缺点,其中植物表达系统具有广阔的发展前景。Objective To review the recombinant expression systems of calcitonin and provide a reference for the further research and development of calcitonin. Methods This paper reviewed the recombinant expression systems of calcitonin on the basis of the published literature. Results At present, there are mainly five expression systems including E. coll. expression system, lactobacillus expression system, yeast expression system, insect baculoviral expression system and plant expression system. Conclusions This review focuses on the advantages and disadvantages in the field of calcitonin expression. Production of calcitonin by plant expression system has a wide developmental foreground.

关 键 词：基因工程 降钙素 外源基因表达系统 

分 类 号：Q789[生物学—分子生物学]