BCNU缓释微球制备条件的优化  

Optimization of preparation of BCNU sustained-release microspheres

作　　者：马晓东[1] 章文斌[1] 周定标[1] 

机构地区：[1]解放军总医院神经外科,北京100853

出　　处：《中华神经外科疾病研究杂志》2006年第4期303-306,共4页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的以溶剂挥发-萃取法制备卡氮芥-聚乳酸羟基乙酸(BCNU-PLGA)缓释微球,对微球制备过程中的工艺条件进行优化选择。方法以正交实验设计对可能的影响因素,包括不同聚合度的聚乙烯醇(PVA)、PVA浓度、PLGA浓度、搅拌速度、甲基纤维素(MC)浓度进行了探讨。结果发现影响微球质量的因素,顺序从大到小依次为:PVA浓度、PLGA浓度、搅拌时间和MC浓度(A-B-D-C)。最佳实验方案为A2B1C2D3,即分散介质PVA浓度为1.5%,有机相中PLGA浓度为2%,搅拌时间为60min,MC浓度为0.05%。结论正交实验设计方法有助于选择更合适的制备条件。Objective The carmustine [ 1-3-bis（2-chloroethyl）-1-nitrosourea, BCNU]-loaded polylacticco-glycolic acid （PLGA） microspheres were fabricated by solvent evaporation and extraction method. The study was to investigate the optimization of the craftwork while the microspheres were synthesized. Methods Determination of the optimal experimental conditions was based on orthogonal design. Possible influential factors including polyvinyl alcohol （PVA） with different degree of polymerization and its concentration, PLGA concentration, agitation rate and methyl cellulose concentration were explored. Results It had been found that the main influential factor was PVA concentration, followed by PLGA concentration, agitation time and methyl cellulose concentration in succession. The most suitable condition was 1.5% of PVA concentration in dispersing phase, 2% of PLGA concentration in organic phase, 60 min of agitation time and 0. 05% of methyl cellulose concentration. Conclusion Orthogonal design is an optimal experiment way for the selection of the appropriate preparation condition.

关 键 词：BCNU 缓释 正交实验设计 PLGA 

分 类 号：R979.1[医药卫生—药品]