干扰素-α联合姜黄素对B-NHL淋巴瘤Raji细胞的增生抑制作用  

Inhibition of proliferation on Faji cells by the combination of INF-α and curcumin

作　　者：吴青[1] 陈燕[1] 李新刚[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,湖北武汉430022

出　　处：《中国癌症杂志》2006年第8期658-661,共4页China Oncology

基　　金：国家科学自然基金课题资助(No:30271672)

摘　　要：背景与目的:姜黄素(curcumin)是中药姜黄的主要成分,其选择性的抑制肿瘤细胞增殖并诱导其凋亡。干扰素-α是目前用于临床治疗最重要的细胞因子之一。本研究旨在探讨干扰素-α联合姜黄素对B-NHL淋巴瘤Raji细胞的诱导凋亡作用及其作用机制。方法:以浓度2000u/L干扰素-α分别与6.25、12.5、25μmol/L浓度的姜黄素作用于体外培养的Raji细胞,观察细胞生长状态变化。MTT法检测细胞生长的抑制率,应用流式细胞仪法观察细胞凋亡,Western blot法检测24h,姜黄素(IC5025μmol/L)以及联合干扰素-α(2000u/L)对caspase6、caspase8、caspase9蛋白的影响。结果:干扰素-α与姜黄素联合应用可显著抑制Raji细胞的生长,诱导细胞发生凋亡,两者具有协同效应。凋亡蛋白caspase6、caspase8、caspase9表达显著增高,其作用呈现出明显的量效与时效关系。结论:干扰素-α与姜黄素联合应用对B-NHL淋巴瘤Raji细胞具有明显的增生抑制作用;促进凋亡蛋白的表达及诱导细胞凋亡,可能是其重要作用机制之一。Background and purpose： Cureumin is the major effective component of eureuma, eureumin has been noted to be able to selectively inhibit proliferation and induce apoptosis in tumor cells. Interferon-α（IFN-α） is one of the most important cytokines used in clinical treatment nowadays. This study was made to investigate the inhibitory effect of interferon-α（IFN-α） and curcumin on Raji cells （B-NHL） and discuss their mechanism. Methods： The variations of morphology of Raji cells were observed in culture medium after being treated by IFN-α（ 500, 100, 2000U/L） combined with various concentrations of curcumin （6.25, 12.5, 25μmol/L）, cells were incubated with the compounds for variable times. The inhibitory ratio was measured by MTT assay, apoptosis was quantitated by flow cytometry（FCM） and the expressions of caspase6, caspase8 and caspase9 in Raji cells were detected by Western blot. Results： both IFN-α and curcumin could significantly induce apoptosis and inhibit the proliferation of Raji cells, synergistic effects were found if cells were treated by the combination of IFN-α and curcumin. The overexpressions of caspase6, caspase8 and caspase9 in Raji cells could be observed in a time-and dose-dependent manner. Conclusions： The combination of IFN-α and curcumin could synergistically inhibit proliferation and induce apoptosis, and its potential mechanism is probably associated with upregulating the expressions of caspase6, caspase8 and caspase9 in B-NHL Raji cell.

关 键 词：干扰素-Α 姜黄素 caspase6 CASPASE8 CASPASE9 细胞凋亡 

分 类 号：R730.51[医药卫生—肿瘤]