Manumycin与DDP、PYM、5-FU、ADR联合的体外抗舌癌作用  

In vitro Anti-cancer Effect of Manumycin Administered in Combination with Cisplatin,Pingyangmycin,5-Fluorouracil or Adriamycin on Carcinoma of the Tongue

作　　者：王葆[1] 梁永钜[1] 符立梧[1] 许光普[1] 

机构地区：[1]华南肿瘤学国家重点实验室中山大学肿瘤防治中心实验研究部,广州市510060

出　　处：《中国肿瘤临床》2006年第15期867-870,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨Manumycin(手霉素)与常用舌癌化疗药物顺铂、5-氟脲嘧啶、平阳霉素、阿霉素联合作用于舌鳞癌Tca8113的细胞毒作用。方法:细胞毒测定以MTT法;单药细胞毒测定以半倍浓度梯度设计;联合用药细胞毒测定以ChouTC中效原理设计。结果:Manumycin以及顺铂、5-氟脲嘧啶、平阳霉素、阿霉素单独应用对Tca8113均有明显的细胞毒作用。联合用药显示在相应比例剂量条件下,Manumycin与顺铂联合(3.75:1)后呈拮抗作用,Manumycin与5-氟脲嘧啶联合(0.75:1)作用后,低浓度联合相互拮抗,而高浓度联合时明显协同,Manumycin与平阳霉素(0.75:1)、阿霉素联合(30:1)作用后呈相互协同的作用(CI<1)。结论:Manumycin与平阳霉素、阿霉素以及与5-氟脲嘧啶高浓度联合作用于Tca8113细胞呈相互协同的细胞毒作用。Objective： To investigate the cytotoxic effect of manumycin combined individually with cisplatin （DDP）, 5-fluorouracil （5-FU）, pingyangmycin （PYM） and adriamycin （ADR） on carcinoma of the tongue. Methods： Cytotoxicity of manumycin was determined by MTT assay, and cytotoxicity of the other drugs alone was determined using the half concentration gradient design. The cytotoxicity of each drug administered in combination with manumycin was determined by the Chou TC lente-principle design. Results： Each drug applied singly showed an obvious cytotoxicity on Tca8113 tongue carcinoma cells. Combined administration of each drug with manumycin showed that with a dosage of corresponding ratio, manumycin produced an antagonism （3.75：1） as combined with Cisplatin, showed a combined mutual antagonism （0.75：1） with a low concentration when combined with 5-Fu and the synergism was overt as with a high concentration. The mutual synergism was shown after joint action of manumycin with Pingyangmycin （0.75：1） and with Adriamycin （30：1） （CI〈I）. Conclusion： The combination of manumycin with PYM and the combination of ADR, manumycin and 5-Fu at high concentration showed synergistic cytotoxicity to Tca8113 cells in vitro （CI〈I）.

关 键 词：MANUMYCIN 联合用药 舌癌 

分 类 号：R739.8[医药卫生—肿瘤]