Fas基因转染对舌鳞癌细胞系Tca8113凋亡的影响  

Study on the Apoptosis Mediated by Fas Gene Transfer in Tca8113 Cell Line

作　　者：王建广[1] 黄洪章[1] 侯劲松[1] 潘朝斌[1] 李劲松[1] 杨斌[1] 程斌[2] 

机构地区：[1]中山大学附属第二医院口腔颌面外科,广东广州510120 [2]中山大学光华口腔医学院口腔内科

出　　处：《广东牙病防治》2006年第3期171-173,共3页Journal of Dental Prevention and Treatment

基　　金：广东省自然科学基金资助项目(A000099013);中山大学科研基金资助项目(B002000007)

摘　　要：目的探讨外源性Fas基因介导舌鳞癌细胞凋亡的可行性及其可能的分子机制。方法脂质体法将重组人Fas真核表达载体转染人舌鳞癌细胞系Tca8113,抗Fas单克隆抗体诱导其凋亡,Westernblotting检测转染前后Tca8113细胞Fas蛋白表达水平,TUNEL法检测转染前后细胞凋亡水平。结果转染后Tca8113细胞Fas蛋白表达上调;抗Fas单克隆抗体可诱导Tca8113细胞凋亡,凋亡指数升高。结论上调Fas基因表达及应用抗Fas单克隆抗体可诱导肿瘤细胞凋亡,有可能作为肿瘤基因治疗的新途径。Objective To investigate the possibility and possible mechanism of apoptosis in tongue squamous cell carcinoma mediated by exogenie Fas gene transfer. Methods Eukaryotic expressing plasmid BK-Fas was reconstructed and transferred into human tongue squamous cell eareinoma cell line Tca8113 by lipofeetamine; anti-Fas monoelonal antibody was used to induee Tca8113 apoptasis, Western blotting assay was applied to detect the expression of Fas protein pre- and post transfection; TUNEL was applied to examine the change of tumor cell apoptasis. Results Up-regulations of F as protein expression were found in Fas transfected eels. Anti-Fas monoelonal antibody could be used in the induction of tumor cell apoptasis. Conclusion Up-regulation of Fas expression can be a new pathway of tumor gene therapy to mediate tumor cell apoptosis.

关 键 词：癌 鳞状上皮 细胞凋亡 FAS基因 基因转染 

分 类 号：R739.86[医药卫生—肿瘤]