海人酸致痫大鼠海马CA_3区神经元线粒体损伤及caspase-3的表达  

Mitochondrial ultrastructural damage and caspase-3 expression in the neurons of hippocampal CA_3 subarea during kainic acid induced status epilepticus in rats

作　　者：汤树海[1] 孙建英[2] 潘晓军[1] 张丽[1] 

机构地区：[1]山东大学齐鲁医院麻醉科,山东济南250012 [2]山东大学齐鲁医院神经内科,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第7期680-683,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2001C10)

摘　　要：目的:观察海人酸(kanic acid,KA)诱导的癫痫持续状态(status epilepticus,SE)大鼠海马CA3区神经元线粒体超微结构的损伤及caspase-3表达的变化。方法:用KA诱导大鼠SE 2 h,分别于SE终止后第3、6、24 h取脑,用电镜观察海马CA3区线粒体的超微结构,同时用免疫组化方法检测相同部位caspase-3表达的变化。结果:SE终止后3 h可见到海马线粒体超微结构损伤,从肿胀到膜的完整性破裂。caspase-3的表达在SE后6 h开始增加,于第24小时明显增高。结论:在实验性SE模型中,海马线粒体的超微结构在早期即有损伤,caspase-3在线粒体损伤后数小时才出现表达,并于SE后24 h明显增高。Objective： To observe the mitochondrial ultrastructure damage and caspase-3 expression in the neurons of hippocampal CA3 subarea during kainie acid（KA） induced status epileptieus（SE）. Methods： SE was induced in adult male Wistar rats with KA, and then 3, 6, and 24 hours later, the brain sections were made. The mitoehondrial ultrastrueture was observed with electron microscope, and the easpase-3 expression was examined by immunohistoehemieal staining. Result： Mitoehondrial ultrastrueture damage could be seen, from swelling to the rupture of membrane 3 hours after SE, whereas easpase-3 expression appeared 6 hours after SE and increased significantly at the 24-hour point after SE. Conclusion： Mitochondrial ultrastrueture is damaged in the early period of the experiment,41 SE rats, and the easpase-3 expression appeared a few hours later.

关 键 词：癫痫持续状态 线粒体超微结构 caspase-3 

分 类 号：R742.1[医药卫生—神经病学与精神病学]