垂体腺瘤VEGF、bFGF和内皮抑素mRNA表达与血管生成的关系  

Expression of VEGF,bFGF,Endostatin and Angiogenesis of Pituitary Adenoma

作　　者：刘凡[1] 王小宜[1] 刘运生[2] 吴光勇[2] 廖伟华[1] 

机构地区：[1]中南大学湘雅医院放射科 [2]中南大学湘雅医院神经外科,湖南长沙410008

出　　处：《医学临床研究》2006年第8期1172-1174,共3页Journal of Clinical Research

基　　金：国家自然科学基金资助项目(30370433)

摘　　要：【目的】检测垂体腺瘤组织中的血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(bF-GF)和内皮抑素mRNA的表达,探讨其与垂体腺瘤血管生成的关系及临床意义。【方法】应用逆转录PCR(RT-PCR)方法检测30例垂体腺瘤组织标本中VEGF、bFGF和内皮抑素mRNA的表达,以Ⅷ因子相关抗原(FⅧ-Rag)血管内皮细胞标记法作为MVD计数的方法,分析上述血管生成相关因子与垂体腺瘤血管生成的关系。【结果】VEGF、bFGF mRNA表达与MVD之间呈显著正相关(r=0.36,r=0.45,均P<0.05),内皮抑素表达和MVD之间无显著相关性(r=-0.17,P>0.05)。【结论】垂体腺瘤血管生成与促血管生成因子VEGF、bFGF基因水平表达的上调有关。[Objective]To detect mRNA expression of vascular endothelial growth factor （VEGF）, basic fibroblast growth factor（bFGF） and endostatin in pituitary adenomas; to explore the relationship between VEGF, bFGF, endostatin mRNA expression and microvessel densilty （MVD） of pituitary adenomas. [Methods] VEGF, bFGF and endostatin mRNA expressions were determined in 30 pituitary adenomas by comparative reverse transcription-polymerase chain reaction （RT-PCR）. MVD was assayed by immunohistochemical staining on factorⅧ related antigen （FⅧ-Rag）to highlight endothelial cells in microvessel . The correlations between mRNA expression of VEGF, bFGF, endostatin and MVD were studied. [Results]The mRNA expression of VEGF and bFGF positively correlated with MVD in pituitary adenomas （ P〈0.05）. Significant correlation was not found between endostatin mRNA expression and MVD. [Conclusion]Up-regulation of VEGF and bFGF expression at the level of gene may play important roles and endostatin may not be critical in the angiogenesis of pituitary adenoma.

关 键 词：垂体肿瘤 内皮生长因子 成纤维细胞生长因子 碱性 RNA 信使 新生血管化 病理性 

分 类 号：R736.4[医药卫生—肿瘤]