牛磺酸对大鼠培养心肌细胞感染病毒后钙离子内流的影响  

EFFECTS OF TAURINE ON CALCIUM INFLUX IN RAT CULTURED CARDIOMYOCYTES AFTER COXSACKIE VIRUS B3 INFECTION

作　　者：宿燕岗[1] 杨英珍[1] 顾全保[2] 郭棋[1] 赵剑星[2] 陈灏珠[1] 

机构地区：[1]上海市心血管病研究所,上海医科大学中山医院,200032 [2]中国科学院上海细胞生物学研究所,200031

出　　处：《上海医科大学学报》1996年第5期339-342,共4页Journal of Fudan University(Medical Science)

摘　　要：观察牛磺酸对心肌细胞感染 ＣｏｘｓａｃｋｉｅＢ３病毒（ＣＶＢ３）后 Ｃａ２＋内流的影响。取新生 ＳＤ大鼠心室肌制备培养搏动心肌细胞， １８ ｈ后接种 １００ ＴＣＩＤ５０的 ＣＶＢ３作为实验性病毒性心肌炎模型，并采用放射性同位素４５Ｃａ２＋示踪技术，观察了 １～２０ ｍｍｏｌ／ Ｌ牛磺酸对正常及感染 ＣＶＢ３后心肌细胞 Ｃａ２＋内流的影响。结果：（ｌ）１０～２０ ｍｍｏｌ／ Ｌ牛磺酸对正常心肌细胞不同胞外 Ｃａ２＋浓度（［Ｃａ２＋］０， ０．５、 １．５和 ４．０ ｍｍｏｌ／ Ｌ）下的 Ｃａ２＋内流均有抑制作用，而 １ｍｍｏｌ／ Ｌ牛磺酸只抑制高［Ｃａ２＋］０（４．０ ｍｍｏｌ／ Ｌ）时 Ｃａ２＋内流，对低［Ｃａ２＋］０（０．５ ｍｍｏｌ／ Ｌ）和正常［Ｃａ２＋］０（１．５ ｍｍｏｌ／ Ｌ）时 Ｃａ２＋内流无明显作用；（２） ｌ～２０ ｍｍｏｌ／ Ｌβ－丙氨酸对 Ｃａ２＋内流无明显影响；（３）感染ＣＶＢ３后Ｃａ２＋内流增加，加用１ｍｍｏｌ／ Ｌ牛磺酸后能减少感染ＣＶＢ３后的Ｃａ２＋内流。结论：牛磺酸具有抑制培养心肌细胞感染ＣＶＢ３后Ｃａ２＋内流的作用，这可能是牛磺酸能减轻ＣＶＢ３所致细胞损伤的机理之一。PURPOSE To investigate the effects of taurine on Ca2+ influx in rat cultured cardiomyocytes after coxsackie virus B3 (CVB3) infection. METHODS Monolayer culture of spontaneously contracting rat heart cells were obtained from newborn SD rats and seeded into culture plates. 100 TCID50 of CVB3 (Nancy strain) was added as the infected group and the effect of l-20 mmol· L-l of taurine and β-alanine, a carboxylic analog of taurine, on Ca2+ influx were detected using radioactive isotope (45Ca2+) tracing techniques. RESULTS (l) 10-20 mmol· L-l of taurine have an inhibition effect on Ca2+ influx in different extracellular calcium concentrations ([Ca2+]0, i.e.: 0.5 mmol/ L, l.5 mmol/ L and 4.0 mmol/ L), I mmol· L-l of taurine had the same effect only when [Ca2+], was at 4.5 mmol/ L and without any effect when [Ca2+], was at l.5 mmol/ L or 0.5 mmol/ L; (2) 1-20 mmol/ L β-alanine have no effect on Ca2+ influx; (3) 1 mmol· L-l of taurine abated significantly the enhancement of Ca2+ influx caused by CVB3. CONCLUSIONS The present results that taurine could inhibit the increased Ca2+ influx after CVB3 attack may be one of the mechanisms of taurine attenuating the cell injury induced by CVB3 infection and probably provide a laboratory support for the clinical use of taurine in viral myocarditis.

关 键 词：牛磺酸 心肌细胞 培养 钙离子内流 

分 类 号：R977.4[医药卫生—药品]