糖皮质激素对人卵巢癌细胞系3AO细胞周期分布及细胞周期蛋白激酶抑制因子p21/WAF1表达的影响  

Effects of glucocorticoids on cell cycle and CDKI p21/WAF1 in a human ovarian cancer cell line:3AO

作　　者：徐明娟[1] 惠宁[1] 刘安[1] 刘宇健[1] 

机构地区：[1]第二军医大学附属长海医院妇产科,上海200433

出　　处：《上海医学》2006年第7期483-485,共3页Shanghai Medical Journal

摘　　要：目的观察糖皮质激素对人卵巢癌细胞系3AO细胞周期分布及细胞周期蛋白激酶抑制因子P21/ WAF1表达的影响。方法采用流式细胞仪技术测定地塞米松(Dex)作用3AO细胞后其细胞周期分布,免疫印迹分析的方法检测Dex对p21／WAF1表达的影响。结果Dex处理后3AO细胞在G_0/G_1期停滞,且这种作用随处理时间延长而更趋明显；同时Dex能够以浓度和时间依赖性方式诱导p21／WAF1表达,这种作用通过GR来介导。结论在GC诱导的抑制细胞增殖的过程中,p21/WAF1的表达增加。Objective To study the effects of glucocorticoids on cell cycle and CDKI p21/WAF1 in a human ovarian cancer cell line：3AO. Methods The cell cycle progression of 3AO after Dex processing was examined with flow cytometry and western blot analysis was used to examine alteration of p21/WAF1 expression under influence of Dex. Results Dexamethasone （Dex） induced G0/G1 arrest of 3AO cells and increased the expression of p21/ WAF1. RU486 reversed the changes of p21/WAF1 expression induced by Dex, which was mediated by GR. Conclusion Up-regulation of p21/WAF1 may contribute to cell proliferation induced by glucocorticoids in 3AO cells.

关 键 词：糖皮质激素 细胞周期 P21/WAF1 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]