TNF基因转染的巨噬细胞细胞毒活性变化的研究  

Cytotoxicity of Macrophages Transfected with TNF Gene

作　　者：曲迅[1] 曹雪涛[1] 于爱莲[1] 李新华[1] 邓文[1] 赵斌华 章卫平 于益芝 蔡绪良 

机构地区：[1]泰山医学院微生物与免疫学教研室,第二军医大学免疫学教研室,潍坊市中心血站

出　　处：《中国肿瘤临床》1996年第10期707-710,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金

摘　　要：将携带ＴＮＦ基因的逆转录病毒上清与小鼠ＭΦ共同孵育，使ＴＮＦ基因导入ＭΦ中，结果发现，ＴＮＦ基因转染的巨噬细胞（ＭΦ─ＴＮＦ＋）分泌ＴＮＦ的水平明显高于正常ＭΦ和转染对照基因的ＭΦ。研究中还发现，ＭΦ─ＴＮＦ＋对Ｐ８１５肿瘤细胞的杀伤率和吞噬力明显高于对照组，用抗ＴＮＦ单抗可明显抑制ＭΦ─ＴＮＦ＋的体外杀伤活性。由此表明，ＭΦ杀伤活性的升高系细胞经基因导入后表达的ＴＮＦ介导的。Murine peritoneal macrophages was transfected by mTNF gene with a retrovirus vector. The TNF gene-transfected macrophages secreted higher level of TNF than the control. The phagocytosis and cytotoxicity of TNF genetransfected macrophages were enhanced significantly, and this enhancement was blocked by anti-TNF monoclonal antibody, denoting that the above augmentation was mediated by TNF gene-transfected macrophages. These data demonstrated that TNF gene-transfected macrophages may be used as a potent effector cells for cancer treatment.

关 键 词：肿瘤 基因治疗 基因转染 肿瘤坏死因子 巨噬细胞 

分 类 号：R730.5[医药卫生—肿瘤]