PPARγ激活剂罗格列酮对人胃癌MGC803细胞周期及其PTEN表达的影响  

Effect of Rosiglitazone on Cell Cycle and PTEN Protein Expression in Human Gastric Carcinoma Cell line MGC803

作　　者：朱理辉[1] 张王利[1] 

机构地区：[1]南华大学附属第二医院消化内科,421001

出　　处：《实用癌症杂志》2006年第3期238-240,共3页The Practical Journal of Cancer

基　　金：湖南省卫生厅资助项目(C2003-015)

摘　　要：目的探讨不同浓度的罗格列酮对人胃癌MGC803细胞增殖及其PTEN蛋白表达的影响。方法不同浓度的罗格列酮(12.5、25、50、100μmol/L)分别与MGC803细胞系共同培养48 h后,采用流式细胞仪检测罗格列酮对MGC803细胞周期的影响,免疫细胞化学检测MGC803细胞PTEN蛋白表达。结果罗格列酮使MGC803细胞停滞于细胞周期G1期,呈剂量依赖性,并诱导PTEN表达上调。结论罗格列酮可以通过诱导PTEN表达上调,使MGC803细胞停滞于细胞周期G1期,进而抑制人胃癌MGC803细胞生长,这可能是PPARγ激活剂抗肿瘤的机制之一。Objective To investigate the influence of rosiglitazone on expression of PTEN in human gastric earcinoma cell line MGC803. Methods Cell cycle of MGC803 cells treated with different concentration, of rosiglitazone for 48h was observed by cytometry,and immunocyte chemistry method was used to examinate the expression of PTEN in MGC803 cells. Results Cell cycle was arrested at Gt stage in a concentration-dependent manner and PTEN protein expres.slon was increased by rosiglitazone. Conclusion One of meachanisms in inhibiting human gastric carcinoma cell line MGC803 was up-regulated expression of PTEN which arrested cell cyle at G1 stage by Rosiglitazone.

关 键 词：罗格列酮 人胃癌MGC803细胞 PTEN蛋白 

分 类 号：R735.2[医药卫生—肿瘤]