HA14-1对白血病细胞的作用  

Effects of HA14-1 on Leukemia Cell Lines

作　　者：薛红漫[1] 李文益[1] 夏焱[1] 

机构地区：[1]广州中山大学附属第二医院儿科,510120

出　　处：《实用癌症杂志》2006年第3期244-246,253,共4页The Practical Journal of Cancer

摘　　要：目的探讨HA14-1对白血病细胞的作用及其与Bcl-2、Bcl-xL和Bax的关系。方法不同浓度HA14-1(25,50,75,100μmol/L)作用于U937、BALL-1和Jurkat clone E6-1细胞4 h,在流式细胞仪上检测作用前后Bcl-2、Bax、Bcl-xL的平均荧光指数(MFI)及作用后细胞凋亡情况。结果①随HA14-1剂量的增加,存活细胞逐渐减少,U937从(93.7±1.5)%降至(14.8±1.9)%;Jurkat clone E6-1从(94.7±1.5)%降至(12.4±1.3)%;BALL-1从(94.7±0.8)%降至(0.9±0.6)%。同一HA14-1剂量时,不同细胞的存活率与Bcl-2表达高低呈负相关,有非常显著性差异(P<0.01)。②随剂量的增加细胞凋亡率逐渐增加,如Jurkatclone E6-1凋亡率从(0.5±0.1)%上升至(60.1±2.6)%;在较高剂量时(100μmol/L),细胞大幅度坏死,坏死率从<(3.3±0.6)%增至(26.8±1.6)%。③HA14-1作用引起3种细胞中Bcl-2表达下降,而HA14-1作用前后Bcl-xL和Bax在3种细胞中均无显著改变。结论HA14-1可以诱导不同Bcl-2表达水平的白血病细胞凋亡,并且细胞对HA14-1的敏感与细胞中Bcl-2表达水平呈相反关系,这为临床逆转Bcl-2高表达造成的化疗耐药提供了理论基础。Objective To investigate the effects of HA14-1 on leukemic cells and the relationship between the effects and the level of Bcl-2、Bcl-xL and Bax in these cells. Methods The leukemic cell lines of U937、BALL-1 and Jurkat clone E6-1 were co- cultured with different concentrations of HA14-1 （25,50,75,100 μmol/L） for 4 hours. The mean fluorescent index （MFI） and the apoptosis of Bcl-2、Bax 、Bcl-xL were detected with the FCM before and after the treatment of HA14-1. Results ①With the increase of concentration of HA14-1, the viability of the cells were decreased from （93.7 ± 1.5） % to （ 14.8 ± 1.9） % in U937 cell; from（94.7± 1.5）% to（12.4 ± 1.3）% in Jurkat clone E6-1; from （94.7 ± 0.8）% to（0.9 ± 0.6）% in BALL-1. With the same HA14-1 concentration, the viability of different cells lines were negatively related with the Bcl-2 level. ②With the increase of HA14- 1 concentration, apoptosis rate increased gradually, such as from（0.5 ± 1.0）% to （60.1 ± 2.6）% in Jurkat clone E6-1. With the high concentration（100 μmol/L） of HA14-1, extreme necrosis was observed . The cell necrotic rate increased from less than（3.3 ± 0.6） % to（26.7 ± 1.6）%. OWith the treatment of HA14-1 ,the Bcl-2 was markedly decreased in all three cells, but the Bcl-xL and Bax were not changed significantly. Conclusion HA14-1 can induce apoptosis of leukemia cells with different Bel-2 level,and the sensitivity of leukemia cells to HA14-1 is opposite to their Bcl-2 expression level ,that is cell with high Bcl-2 level is more sensitive to HA14-1,which offers theoretical basis for reversing MDR clinically.

关 键 词：白血病 细胞凋亡 BCL-2蛋白 

分 类 号：R733.7[医药卫生—肿瘤]