DNA倍体分析在恶性腹腔积液诊断中的价值  

Diagnosis Value of DNA Ploid Detection in Malignant Ascites

作　　者：卜照香[1] 陈文学[1] 

机构地区：[1]江西省肿瘤医院,330029

出　　处：《实用癌症杂志》2006年第3期300-301,共2页The Practical Journal of Cancer

摘　　要：目的探讨DNA异倍体对于恶性腹腔积液的诊断价值。方法抽取64例中等量以上腹腔积液患者的腹水,分离其中的细胞,制备单细胞悬液,应用流式细胞仪分析DNA异倍体。结果DNA异倍体在肿瘤细胞中出现率(82.7%)显著高于非肿瘤细胞(0),统计学检验表明两者有显著性差异。结论流式细胞术分析腹水细胞的DNA异倍体,对于恶性肿瘤的细胞学诊断有较大的意义,可在临床推广应用。Objective To discuss the diagnosis value of DNA ploid detection in malignant ascites. Methods To obtain the aseitcs from 64 cascs suffered from the medium or large volume of aseites. The DNA ploid of cells were separated from aseites was detected by flow cytometry. To investigate the relation of DNA ploid and the diagonosis of cytology and clinic. Results The rates of DNA aneuploid in the tumor group（82.7% ） was higher than that of normal cell group（0）. The difference was significant. Conclusion DNA aneuploid of ascites detected by flow cytometry provides valuable cytodiagnosis for the malignant tumor. It should be applied in clinical laboratory.

关 键 词：流式细胞仪 DNA异倍体 诊断 

分 类 号：R730.43[医药卫生—肿瘤]