大鼠海人藻酸致痫时海马内一氧化氮合成酶mRNA含量的时程变化  

COURSE OF NITRIC OXIDE SYNTHASE mRNA ALTERATIONS DURING KAINIC ACID-INDUCED EPILEPSY IN RAT HIPPOCAMPUS

作　　者：王布尔[1] 程介士[1] 

机构地区：[1]医学神经生物学国家重点实验室,上海医科大学基础医学院神经生物学教研室,200032

出　　处：《上海医科大学学报》1996年第5期373-374,共2页Journal of Fudan University(Medical Science)

基　　金：国家自然科学基金!(3940003)资助课题

摘　　要：观察海马内一氧化氮合成酶 ｍＲＮＡ（ＮＯＳ ｍＲＮＡ）在海人藻酸（ｋａｉｎｉｃ ａｃｉｄ， ＫＡ）致病时的时程变化。用Ｎｏｒｔｈｅｒｎ印迹法分别观察用药前及致痫后１，４，６，１２，２４ ｈ海马内ＮＯＳ ｍＲＮＡ的含量变化。结果：致痫后１ｈ，ＮＯＳ　ｍＲＮＡ即显著增加，并在以后癫痫连续发作的２４ｈ中一直维持这种高含量。结论：ＫＡ致病可能与海马ＮＯＳ ｍＲＮＡ含量变化相关。PURPOSE To investigated the time course of nitric oxide synthase (NOS) mRNA alterations during kainic acid (KA)-induced epilepsy in rat hippocampus. METHODS Using Northern blot, hippocampal NOS mRNA contents were measured before adminitration and at l, 4, 6, 12, 24 hour respectively after onset of epileptic activity evoked by KA (12 mg/ kg). RESULTS 1 hour after initiating epilepsy, the hippocampal NOS mRNA levels increased to 300 % of the control, and stayed at this high level during the rest of 24 hours. CONCLUSIONS KA-induced epilepsy may be related to the changes of hippocampal NOS mRNA level.

关 键 词：癫痫 海人藻酸 海马 一氧化氮合成酶 mRNA 

分 类 号：R742.102[医药卫生—神经病学与精神病学]