芦荟库拉索(Aloe vera L)再生体系的建立  

Establishment of Plant Regeneration System for Aloe vera L.

作　　者：吴英[1] 郭安平[1] 孔华[1] 贺立卡[1] 郭运玲[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术国家重点实验室

出　　处：《热带作物学报》2006年第2期55-59,共5页Chinese Journal of Tropical Crops

摘　　要：对芦荟(AloeveraL.)组织培养和再生体系进行了研究,初步获得芦荟的初代和继代培养基为MS+6-BA2.5mg/L+NAA0.2mg/L+蔗糖30mg/L,生根培养基成分为1/2MS+IBA0.2mg/L+NAA0.1mg/L+蔗糖30mg/L,愈伤组织培养采用粗壮的芦荟基部的白色部分,诱导愈伤组织的培养基为B5+6-BA2.0mg/L+2,4-D2.0mg/L+蔗糖30mg/L,采用Vc5.0mg/L抑制愈伤组织诱导过程中褐变的产生。诱导愈伤组织和外植体直接出芽2种方式并存。试管苗移栽后存活率为100%。采用潮霉素为抗性筛选标记,选择浓度为20￣30mg/L。A study was made on tissue culture and plant regeneration of Aloe vera L.. The results showed that the optimum media were MS＋6-BA 2.0 mg/L＋2,4-D 2.0 mg/L＋sucrose 30 mg/L for inducing calli; MS＋6-BA2.5 mg/L＋ NAA 0.2 mg/L＋sucrose 30 mg/L for inducing cluster shoots; 1/2MS＋IBA 0.2 mg/L＋NAA 0.1 mg/L＋sucrose 30 mg/L for rooting. Browning was inhibited by using Vc 5.0 mg/L in the course of callus inducing. The survival rate of the tube plantlets transplanted was 100 %. Hygromycin was used as a mark for selecting the stress-resistant plant at a concentration of 20-30 mg/L.

关 键 词：库拉索芦荟 诱导出芽 诱导生根 诱导愈伤组织 褐变 

分 类 号：S667.903.6[农业科学—果树学]