突变型P53蛋白在128例HCC中的表达  

Expression of four anti-P53 proteins in 128 cases of hepatocellular carcinoma

作　　者：倪灿荣[1] 戴益民[1] 张松平[1] 

机构地区：[1]第二军医大学基础医学部病理解剖学教研室,无锡市第四人民医院病理科

出　　处：《第二军医大学学报》1996年第4期336-339,共4页Academic Journal of Second Military Medical University

摘　　要：目的：研究Ｐ５３蛋白在肿瘤组织中的表达。方法：选择４种抗突变型Ｐ５３蛋白抗体，应用免疫金银染色法检测１２８例人肝细胞癌（ＨＣＣ）及癌旁组织的表达。结果：Ｐ５３阳性表达总体阳性率为７４．２％（９５／１２８），其中ＰＡｂ１８０１和ＣＭ－１阳性率最高，分别为６９．５３％（８９／１２８）和６７．１８％（８６／１２８），ＤＯ－７为３９．８％（５１／１２８），ＰＡｂ２４０最低，为１９．５３％（２５／１２８）。４６例癌旁组织也有不同程度的阳性表达。ＣＭ－１主要在核内表达，而其他３种Ｐ５３蛋白表达有异质性，但仍以核内表达为主。Ｐ５３蛋白的阳性表达与组织学分级、肿瘤预后复发及肿瘤大小关系密切（Ｐ＜０．０５），但与组织学类型无相关性（Ｐ＞０．０５）。结论：Ｐ５３基因突变在肝癌进展的早期和晚期均有表达，不一定是晚期事件，作为肿瘤的侵袭表型，可能在肝癌发生的早期业已存在。Ｐ５３的高表达与乙型肝炎病毒感染关系密切（Ｐ＜０．０１），Ｐ５３的阳性表达存在地理差异。ＣＭ－１和ＰＡｂ１８０１抗体检测ＨＣＣ具有代表性，但４种Ｐ５３蛋白同时检测更能全面反映ＨＣＣ中Ｐ５３蛋白的累积情况。Objective:To detect the expression of mutation P53 protein in the human hepatocellular carcinoma tissue.Methods:The expression of mutation P53 protein was observed in the human hepatocellular carcinoma tissue and the tissue nearby carcinoma with immunogold silver staining method in 128 cases.Results:The expression of P53 in 95 cases was positive and the total positive rate 74.2%(95/128).The positive rates of PAb1801(69.53%,89/128) and CM-1(67.18%,86/128) were higher than that of DO-7(39.8%,51/128) and PAb240(19.53%,25/128).The expression of mutation P53 in the tissue nearby carcinoma was also positive to some extent in 46 cases.CM-1 appeared mainly in nuclei,and the others were observed in nuclei,cytoplasma or cell membrane with heterogeneity,but mainly in nuclei.The expression of mutation P53 protein was related closely to histopathologic grade,tumor size,metastasis and prognosis of tumor(P<0.05),but it was not related to histologic types(P>0.05).Conclusion:The mutation of P53 gene might appear in both early and advanced stages of hepatocellular carcinoma,not certainly be a later event.And as an invasiveness-type,it is possible to exist in early stage of hepatocellular carcinoma.The high expression of mutation P53 is related closely to hepatitis B virus infection(P<0.01).There is a geographical divergence in the expression.The positive results of CM-1 and PAb1801 are typical.Nevertheless,it would give us a fuller view on the accumulation of mutation P53 proteins to detect P53 with the all four kinds of antibodies.

关 键 词：肝肿瘤 P53蛋白 免疫组织化学 

分 类 号：R735.702[医药卫生—肿瘤]