低分子RNA与高三尖杉酯碱联用对白血病细胞系HL-60细胞作用的实验观察  

Experimental observation of low-molecular RNA and homoharringtonine on leukemic HL-60 cells proliferation

作　　者：刘影[1] 方基勇[2] 吴明彩[2] 毕富勇[2] 

机构地区：[1]皖南医学院医学系,安徽芜湖241001 [2]皖南医学院生物化学教研室,安徽芜湖241001

出　　处：《皖南医学院学报》2006年第3期167-169,共3页Journal of Wannan Medical College

基　　金：院中青年科研基金项目(WK200407A)

摘　　要：目的：研究低分子RNA与抗白血病药物高三尖杉酯碱联用对HL-60细胞药物敏感性的影响。方法：采用细胞形态学观察、四唑盐（MTT）比色法分析细胞增殖以及应用凯氏定氮法测定细胞蛋白质的含量。结果：①0．05～50μg／ml高三尖杉酯碱对HL-60细胞增殖有抑制作用，并与时间和剂量相关。②低分子RNA与高三尖杉酯碱联用能显著降低HL-60细胞的增殖率，并进一步降低细胞内蛋白质的合成。结论：低分子RNA能提高HL-60细胞对白血病药物高三尖杉酯碱的敏感性，两者联用能有效抑制白血病细胞系HL-60细胞的增殖。Objective： To investigate the effects of low-molecular RNA and antileukemic drug homoharringtonine on drug-sensitivity of HL- 60 cells. Methods ： By cell morphological observation, cell proliferation was examined by employing cell morphological observation and MTT assay technique and determination of cell protein level was carried out by Kjeldahl＇s method. Results ： （ 1 ）0.05 - 50 μg/ml homoharringtonine had obvious inhibitory effect on the cell proliferation of HL-60 cells and the inhibitory effect correlated with time duration and dosage. （2）Lowmolecular RNA could intensify the inhibitory effect of homoharringtonine on the cell proliferation of HL-60 cells. Conclusion ： Low-molecular RNA can improve drug-sensitivity of Leukemic HL-60 cells to homoharringtonine. Combined administration of low-molecular RNA and homoharringtonine can effectively inhibit the cell proliferation of Leukemic HL-60 cells.

关 键 词：低分子RNA HL-60细胞 高三尖杉酯碱 药物敏感 白血病 

分 类 号：R733.7[医药卫生—肿瘤]