肝细胞生长因子对后肢固定大鼠腓肠肌细胞凋亡的影响  

Effect of hepatocyte growth factor on the apoptosis of gastrocnemius cell of fixed hindlimb in rats

作　　者：王东[1] 余斌[1] 朱杰[1] 黄炎[1] 

机构地区：[1]南方医科大学南方医院创伤骨科,广东广州510515

出　　处：《西北国防医学杂志》2006年第4期285-287,F0003,共4页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:观察肝细胞生长因子对后肢固定大鼠腓肠肌细胞凋亡的影响。方法:按照Jozsa的方法制作模型,实验组将肝细胞生长因子注射入大鼠腓肠肌;模型组注射等量生理盐水。观察骨骼肌组织的病理改变及骨骼肌细胞凋亡的改变。结果:实验组肌纤维塑形良好,线粒体形态较正常,见新生肌纤维;Bc1-2呈强阳性表达且TUNEL显示为低表达,均较模型组明显改变(P<0.01)。结论:肝细胞生长因子能够显著降低骨骼肌细胞凋亡。Objective ：To observe the effect of hepatocyte growth factor （HCF） on the apoptosis of gastrocnemius ceUs of fixed hindlimb in rats. Methods： Animal models were made in rats by Jozsa method. HGF （10 mg/kg） was injected into gastrocnemius of rats in experiment group, and normal saline was injected in control group. The pathological changes and the apoptosis of skeletal muscle cells were observed and recorded. Results： The muscle fibers were good in shape, the shape of bioblast was normal, and new born muscle fibers could be found in the experiment group. TUNEL assay showed that the level of positive expression was higher in the control group than that in the experiment group （ P 〈 0.01 ）. The level of positive bcl - 2 expression was higher in the experiment group than that in the control group （ P 〈 0.01 ）. Conclusion： HGF can obviously decrease the apoptosis of skeletal muscle cells, and it has a preventive effect on disuse atrophy of skeletal muscle.

关 键 词：肝细胞生长因子 废用性萎缩 骨骼肌 凋亡 

分 类 号：R363[医药卫生—病理学]