胃癌组织芯片p53、p16及环氧合酶-2表达的研究  

Expression of tissue microarray p53,p16 and cyclooxygenase-2 in gastric cancer

作　　者：虞朝辉[1] 李岚[1] 厉有名[1] 张宝峰[2] 方静[2] 周琼[2] 胡莺[2] 郜恒骏[2] 

机构地区：[1]浙江大学医学院附属第一医院消化科,杭州310003 [2]生物芯片上海国家工程研究中心

出　　处：《中华内科杂志》2006年第8期658-660,共3页Chinese Journal of Internal Medicine

基　　金：国家863计划功能基因组与生物芯片重大专项资助(2002AA222021);浙江省科技发展计划资助(2005C3002)

摘　　要：目的利用高通量的组织芯片技术,对胃癌组织及癌旁组织的p53、p16和环氧合酶-2 (COX-2)蛋白异常表达进行分析,探讨其相关性及临床意义。方法利用组织芯片技术结合免疫组化法检测50例胃癌组织和78例癌旁组织中p53、p16及COX-2蛋白的表达。结果p53、p16和COX-2的阳性表达率在癌旁组织中分别是19%、15%及74%；在癌组织中分别是50%、54%及94%。胃癌组织中p53、p16和COX-2蛋白表达均显著高于癌旁组织,差异具有统计学意义(P＜0．05)。p53与COX-2、p16与COX-2蛋白表达均存在相关性(P＜0．05)。当p53、COX-2表达阳性,p16阴性时；或p16、COX-2表达阳性,p53阴性时；或p53、p16和COX-2同时表达阳性时,组织芯片病理类型为癌性的概率均增加,OR值分别为11．667、30．000及18．889,p53、p16和COX-2三者存在交互作用。结论联合分析p53、p16及COX-2的表达对预测胃癌的发生和发展具有重要意义。Objective To constructed tissue microarray containing specimens from gastric cancer and adjacent non-cancer tissue to survey the expression of p53, p16 and cyclooxygenase-2（COX-2） , and to obtain a comprehensive survey on the expression of three proteins in gastric cancer progression and their clinical significance. Methods We constructed a tissue microarray containing 50 specimens from gastric cancer and 78 specimens from adjacent non-cancer tissue, and assayed three different proteins （ p53, p16 and COX-2） by immunohistochemistry to consecutive formalin-fixed tissue microarray sections. Results In non-cancer tissue, p53, p16 and COX-2 positive staining were 19% , 15% and 74% respectively. In gastric cancer tissue, p53, p16 and COX-2 positive staining were 50%, 54% and 94% respectively. There were a significant difference between two different tissues （P 〈0. 05 ）. 52% （66/128） cases were COX-2 ＋/p53 -, only 1 case were COX-2 -/p53 ＋. There was a significant association between COX-2 and p53 （P 〈 0. 05 ）. COX-2 negative expression tissues were found significantly more often in p53-negative than in p53- positive cases. 52% （67/128） cases were COX-2 ＋/p16 -, only 1 case were COX-2 -/p16 ＋. A significant correlation was recognized between expression of COX-2 and p16 （P 〈 0. 05）. Logistic regression analysis revealed that the risk factors of tumorous histotype were p53, p16 and COX-2 positive expression together, determined by Wald χ^2 test （P 〈0. 05; odds ratio, 18. 889）. There was reciprocal relationship among p53, p16 and COX-2. Conclusion Combination analysis of p53, p16 and COX-2 may be useful for the prediction of gastric carcinogenesis.

关 键 词：胃肿瘤 蛋白质P53 蛋白质P16 组织芯片 

分 类 号：R735.2[医药卫生—肿瘤]