复发性阿弗他溃疡患者转录因子T-bet和GATA-3失衡表达的研究  

Expression of T cell-specific transcription factors T-bet and GATA-3 in recurrent aphthous ulcerations

作　　者：朱金晓[1] 单莉英[1] 李莉华[2] 黄朝辉[2] 刘阿贵[1] 姚远[1] 

机构地区：[1]苏州大学附属无锡市第四医院口腔科,江苏214062 [2]苏州大学附属无锡市第四医院,中心实验室,江苏214062

出　　处：《中华口腔医学杂志》2006年第8期494-497,共4页Chinese Journal of Stomatology

基　　金：江苏省卫生厅科研指导性课题基金(Z200516)

摘　　要：目的探讨转录因子T-bet和GATA-3在复发性阿弗他溃疡(RAU)免疫失衡中的意义。方法密度梯度离心法分离RAU患者和对照组外周血单个核细胞,酶联免疫吸附测定法(ELISA)检测RAU患者和对照组血清中白细胞介素(IL)-4和γ-干扰素(γ-IFN)的含量；逆转录-聚合酶链反应(RT-PCR)测定法和Western blot法分别检测RAU患者和对照组外周血单个核细胞中T-bet和GATA-3 mRNA和蛋白的表达。结果对照组和RAU患者外周血单个核细胞中,T-bet mRNA表达分别为0.73±0.14和0.12±0.04,T-bet蛋白表达分别为0.73±0.18和0.24±0.09,两组比较差异均有统计学意义(P＜0.01)；GATA-3 mRNA表达分别为0.89±0.11和1.30±0.13,GATA-3蛋白分别为1.09±0.16和1.80±0.16,两组比较差异均有统计学意义(P＜0.01)；血清中γ-IFN的含量对照组(22.15±1.52)ng/L明显高于RAU组(16.32±0.22)ng/L(P＜0.01)；IL-4含量对照组(25.58±2.59)ng/L明显低于RAU组(43.60±3.15)ng/L(P＜0.01)；γ-IFN/IL-4比值与T-bet/GATA-3 mRNA和蛋白表达量的比值均呈正相关(r=0.96、0.94,P＜0.01)。结论转录因子T-bet/GATA-3失衡表达是RAU免疫失衡的重要原因之一。Objective To investigate the expression of the T cell-specific transcription factors T-bet/GATA-3 in recurrent aphthous ulcerations （RAU）. Methods Peripheral blood mononuclear cells （PBMC） were isolated by density gradient centrifugation from RAU patients and healthy controls. Expressions of T-bet and GATA-3 mRNA and protein in the PBMCs were detected by reverse transcriptionpolymerase chain reaction （RT-PCR） and Western blot respectively. The concentrations of γ-interferon （γ-IFN） and interleukin-4 （IL-4） in the serum were determined by enzyme-linked immunosorbent assay （ELISA）. Results The expressions of T-bet mRNA were 0. 73 ± 0. 14 in the control group and 0. 12 ± 0. 04 in the recurrent aphthous ulcerations group, the levels of T-bet protein expressions were 0. 73 ± 0. 18 in the control group and 0. 24± 0. 09 in the recurrent aphthous ulcerations group, there was a significant difference between the two groups （P 〈 0. 01 ）. The levels of GATA-3 mRNA expressions were 0. 89 ± 0. 11 in the control group and 1.30 ±0. 13 in the recurrent aphthous ulcerations group; GATA-3 protein expressions were 1.09± 0. 16 in the control group and 1.80 ± 0. 16 in the recurrent aphthous ulcerations group, there was a significant difference between the two groups（P 〈0.01 ）. In the control group, the concentration of γ-interferon in the serum was （22. 15 ± 1.52）ng/L , which was significantly elevated compared with that in the recurrent aphthous ulcerations group （ 16. 32 ±0. 22） ng/L （ P 〈 0. 01 ）, in the control group, the concentration of IL-4 in the serum was（25.58 ±2. 59） ng/L, which was significantly lower than that of the recurrent aphthous ulcerations group（43.60 ± 3.15 ） ng/L（ P 〈 0.01 ）. The ratio of γ-IFN and IL-4 was positively correlated with the ratio of T-bet and GATA-3 mRNA and protein expression （ r =0. 96,0. 94, P 〈 0. 01 ）. Conclusions Imbalance of transcription factors T-bet and GATA-3 may be one of the key factors in immune dys

关 键 词：转录因子 T淋巴细胞 复发性阿弗他溃疡 

分 类 号：R781.5[医药卫生—口腔医学]