微丝对牵张应变诱导的人牙周膜成纤维细胞中环氧合酶-2表达的影响  

Tension-force Induced Cyclooxygenase-2 Expression Mediated by Microfilament in Human Periodontal Ligament Fibroblast

作　　者：熊培颖[1] 黄生高[1] 张建兴[1] 

机构地区：[1]中南大学湘雅二医院口腔科,湖南长沙410011

出　　处：《华西口腔医学杂志》2006年第4期353-356,共4页West China Journal of Stomatology

基　　金：湖南省自然科学基金资助项目(03jjy4033)

摘　　要：目的探讨微丝的聚合在人牙周膜成纤维细胞(hPDLFs)正畸力信号传导中的作用。方法将培养的hPDLFs接种于膜式细胞牵张应变施加装置上,随机分为对照组、单纯加力组、加力+细胞松弛素组。对照组牵张应变量为0;单纯加力组和加力+细胞松弛素组设定细胞静态牵张应变量为18%,加载时间分别为8h、16h和24h。加力+细胞松弛素组在施加牵张应变量前加入5μg/mL的细胞松弛素B来破坏微丝的聚集作用。用免疫组织化学法检测细胞中环氧合酶-(2COX-2)的表达,测定灰度值并进行统计学处理。结果单纯加力组在18%牵张应变量作用下,COX-2表达随着加载时间的增加而增强;加力+细胞松弛素组在18%牵张应变量作用下,COX-2的表达明显降低,且各加载时间组间表达无差别。结论微丝的聚合介导了hPDLFs中正畸力信号传导,破坏微丝的聚合作用将阻碍力学信号的传导,从而降低COX-2的表达。Objective To study the role of microfilament polymerization in menchanotransduction by human periodontal ligament fibroblast （.hPDLFs）. Methods In tension-force group, hPDLFs were treated by tension-force values of 18% for 8 h, 16 h, 24 h. In tension-force and inhibitor group, the sample was treated with 5μg/mL cytochalasin B before using tension-forece. Each sample was collected and the expression of cyclooxygenase-2 was measured by using immunohistoche staining. Results In tension-force group, the expression of cyclooxygenase-2 enhanced with the extension of loading time. In tension-force and inhibitor group, cyclooxygenase-2 expression was depressed and had no relation with loading time. Condnsion Tension-force induced cyclooxygenase-2 expression is mediated by microfilament, disruption of the microfilament polymerization will destroy mechanotransduction in hPDLFs.

关 键 词：人牙周膜成纤维细胞 环氧合酶-2 力信号传导 微丝 

分 类 号：R780.2[医药卫生—口腔医学]